"New technique for quantitative elelectrochemical determination of total plasma mRNA"@en . "54" . . "Adam, Vojt\u011Bch" . "The aim of this work was to propose new technique for isolation and detection of total content of mRNA in human plasma. The total content of plasma mRNA varied from 0.1 to 1.2 ug/ml. We succeeded in isolating mRNA by using simple technique with MPs. Recovery of the isolation of mRNA (tested on polyA chains) was about 30 %. In comparison with common techniques used for isolation of mRNA (e.g. phenol-chloroform extraction) the recovery of the MPs approach is higher and, moreover, this technique is not so time consuming, because we were able to analyse twelve plasma samples within 120 minutes."@en . "4"^^ . . . . . "Kizek, Ren\u00E9" . "New technique for quantitative elelectrochemical determination of total plasma mRNA" . . . "Pr\u016F\u0161a, Richard" . "Nov\u00E9 techniky pro kvantitativn\u00ED elektrochemick\u00E9 stanoven\u00ED celkov\u00E9 plazmatick\u00E9 mRNA"@cs . "7"^^ . "43210" . "382676" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "New technique for quantitative elelectrochemical determination of total plasma mRNA"@en . . . "P(KAN208130801)" . "RIV/62156489:43210/08:00133057!RIV09-AV0-43210___" . "H\u00FAska, Dalibor" . "The aim of this work was to propose new technique for isolation and detection of total content of mRNA in human plasma. The total content of plasma mRNA varied from 0.1 to 1.2 ug/ml. We succeeded in isolating mRNA by using simple technique with MPs. Recovery of the isolation of mRNA (tested on polyA chains) was about 30 %. In comparison with common techniques used for isolation of mRNA (e.g. phenol-chloroform extraction) the recovery of the MPs approach is higher and, moreover, this technique is not so time consuming, because we were able to analyse twelve plasma samples within 120 minutes." . . "Vajtr, D." . . . "Kukacka, J." . "Alba Mej\u00EDa, Jhonny Edison" . "New technique for quantitative elelectrochemical determination of total plasma mRNA" . "mRNA"@en . "RIV/62156489:43210/08:00133057" . "6" . "2"^^ . "C\u00EDl t\u00E9to pr\u00E1ce byl vyvynout novou techniku pro isolaci a detekci celkov\u00E9 mRNA v lidsk\u00E9 plazm\u011B. elkov\u00FD obsah mRNA v plazm\u00E9 se pohybovala v rozmez\u00ED 0.1 a\u017E 1.2 ug/ml. V\u00FDt\u011B\u017Enost izola\u010Dn\u00ED metody dosahoval kolem 30 %. V porovn\u00E1n\u00ED s klasick\u00FDmi izola\u010Dn\u00EDmi postupy chloroform-fenol m\u011Bl pou\u017Eit\u00FD postup vy\u0161\u0161\u00ED v\u00FDt\u011B\u017Enost a rovn\u011B\u017E byl i rychlej\u0161\u00ED."@cs . . . "Nov\u00E9 techniky pro kvantitativn\u00ED elektrochemick\u00E9 stanoven\u00ED celkov\u00E9 plazmatick\u00E9 mRNA"@cs . "Clinical chemistry" . . "0009-9147" . "[EFC03EEEE14D]" .