"8"^^ . . . . "Rauscherov\u00E1, L." . "Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry"@en . "12"^^ . . "platin based cytostatic"@en . . "Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry" . "Bioelectrochemistry" . "CH - \u0160v\u00FDcarsk\u00E1 konfederace" . "72" . "Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry"@en . . . . "Pikula, Ji\u0159\u00ED" . "Vliv cisplatiny na vodn\u00ED rostlinu Lemna minor tudovan\u00FD voltametri\u00ED"@cs . "Zehn\u00E1lek, Josef" . "Singer, Christoph" . . . "Baloun, Ji\u0159\u00ED" . "In the present work we report on investigation of affecting of duckweed (Lemna minor) by various doses of cisplatin (0, 5, 10, 20, 40, 80 and 160 uM) for 4 days. The toxic influence of cisplatin was evaluated on the basis of growth inhibition expressed as number of leaves, growth rate, and total amount of biomass. The result value of 96hEC50, calculated from growth inhibition with comparison of growth rates, was 6.93 uM. Moreover we aimed on determination of cisplatin content using differential pulse voltammetry. The highest content of cisplatin (320 ng g-1 of fresh weight) was determined in plants treated by 80 uM at the second day of treatment. Plants protect themselves against heavy metals by means of synthesis of cysteine-rich peptides such as glutathione and phytochelatins. Thus thiol determination in the treated plants by means of Brdicka reaction followed. The marked increase in thiol concentration detected is associated with defence reaction of the plant against stress caused by cisplatin." . "Vliv cisplatiny na vodn\u00ED rostlinu Lemna minor tudovan\u00FD voltametri\u00ED"@cs . "V t\u00E9to pr\u00E1ci jsme zkoumli vliv r\u016Fzn\u00FDch d\u00E1vek cis-platiny (0-160uM) ma Lemna minor po dobu 4 dn\u016F. Toxick\u00FD vliv cis-platiny byl zji\u0161t\u011Bn na z\u00E1klad\u011B r\u016Fstov\u00E9 inhibice vyj\u00E1d\u0159en\u00E9 v podob\u011B po\u010Dtu list\u016F a r\u016Fstov\u00E9ho pom\u011Bru byiomasy. Zji\u0161t\u011Bn\u00E1 hodnota pro pom\u011Br tvorby biomasy EC 50 po 96 hodin\u00E1ch byla 6.93 uM. Mimoto jsem zjistliy obsah cysplatiny pomoc\u00ED diferen\u010Dn\u00ED pulsn\u00ED voltametrie. Nejvyssi obsah byl cis-platiny 320 ng/g \u010Derstv\u00E9 hmotnosti byl zji\u0161t\u011Bn u rostlin vystaven\u00FDch 80 uM ve druh\u00E9m dnu experimentu. Rostlinn\u00E1 chrana zahrnovala i tvorbu na cystein bohat\u00FDch slou\u010Debnin."@cs . "Kizek, Ren\u00E9" . "Adam, Vojt\u011Bch" . "Affecting of aquatic vascular plant Lemna minor by cisplatin revealed by voltammetry" . "RIV/62156489:43210/08:00122083!RIV09-MSM-43210___" . . . . "P(1M06030), P(GA522/07/0692), Z(MSM6215712402)" . "In the present work we report on investigation of affecting of duckweed (Lemna minor) by various doses of cisplatin (0, 5, 10, 20, 40, 80 and 160 uM) for 4 days. The toxic influence of cisplatin was evaluated on the basis of growth inhibition expressed as number of leaves, growth rate, and total amount of biomass. The result value of 96hEC50, calculated from growth inhibition with comparison of growth rates, was 6.93 uM. Moreover we aimed on determination of cisplatin content using differential pulse voltammetry. The highest content of cisplatin (320 ng g-1 of fresh weight) was determined in plants treated by 80 uM at the second day of treatment. Plants protect themselves against heavy metals by means of synthesis of cysteine-rich peptides such as glutathione and phytochelatins. Thus thiol determination in the treated plants by means of Brdicka reaction followed. The marked increase in thiol concentration detected is associated with defence reaction of the plant against stress caused by cisplatin."@en . "Beklov\u00E1, Miroslava" . . . "Havel, Ladislav" . . . . "Sures, Bernd" . "\u0160up\u00E1lkov\u00E1, Veronika" . "H\u00FAska, Dalibor" . "1" . . "[06C202838C87]" . "RIV/62156489:43210/08:00122083" . . "1567-5394" . "355060" . . "43210" . "7"^^ . . .