"[7B7DF2E7FE08]" . "Macek, Tom\u00E1\u0161" . . "V t\u00E9to pr\u00E1ci jsme se zam\u011B\u0159ili na zkoum\u00E1n\u00ED transgenn\u00EDch rostlin exprimuj\u00EDc\u00EDch metalothionein. Jedna z \u00FAloh tohoto proteinu v \u017Eivo\u010Di\u0161n\u00E9m organismu je ochrana p\u0159ed toxick\u00FDmi koncentracemi t\u011B\u017Ek\u00FDch kov\u016F. U rostlin tuto funkci zast\u00E1vaj\u00ED fytochelatiny -- peptidy bohat\u00E9 na cystein. Spolu\u00FA\u010Dast obou mechanizm\u016F u modifikovan\u00FDch rostlin zvy\u0161uje jejich toleranci k t\u011B\u017Ek\u00FDm kov\u016Fm. Tato skute\u010Dnost m\u016F\u017Ee b\u00FDt vyu\u017Eita ve fytoremedia\u010Dn\u00EDch technologi\u00EDch, kde rostliny s vysokou akumula\u010Dn\u00ED schopnost\u00ED sni\u017Euj\u00ED n\u00E1klady a efektivitu dekontaminace. Stanovili jsme obsah transgenn\u00EDho metalothionein u r\u016Fzn\u00FDch klon\u016F tab\u00E1ku a porovnali ho z experiment\u00E1ln\u011B zji\u0161t\u011Bnou akumula\u010Dn\u00ED schopnost\u00ED rostlin. Z\u00E1rove\u0148 jsme stanovili obsah celkov\u00FDch thiol\u016F a obsah redukovan\u00E9ho a oxidovan\u00E9ho glutathionu, fytochelatinu 2 a cysteinu, dal\u0161\u00EDch slou\u010Denin pod\u00EDlej\u00EDc\u00EDch se na detoxikaci t\u011B\u017Ek\u00FDch kov\u016F. Prok\u00E1zali jsme v\u00FDraznou expresi transgenn\u00EDho metalothioneinu, kter\u00FD m\u011Bl nejv\u011Bt\u0161\u00ED pod\u00EDl na zji\u0161t\u011Bn\u00E9 vy\u0161\u0161\u00ED akumula\u010Dn\u00ED schopnost\u00ED transgenn\u00EDch rostlin tab\u00E1ku." . "Shestivska, Violetta" . . . . "metallothionein; transgenic plants; low-molecular thiols"@en . "Kizek, Ren\u00E9" . . "43210" . . . "Zehn\u00E1lek, Josef" . "Adam, Vojt\u011Bch" . "Diopan, V\u00E1clav" . . . . "5"^^ . "RIV/62156489:43210/08:00121964" . "Stanoven\u00ED metalothioneinu u transgenn\u00EDch rostlin s potenci\u00E1lem vyu\u017Eit\u00ED ve fytoremedia\u010Dn\u00EDch technologi\u00EDch t\u011B\u017Ek\u00FDch kov\u016F"@cs . "RIV/62156489:43210/08:00121964!RIV09-MSM-43210___" . . . "Praha" . . . "Determination of metallothionein at transgenic plants - Using for heavy metal phytoremediation technologies"@en . "P(1M06030)" . "Stanoven\u00ED metalothioneinu u transgenn\u00EDch rostlin s potenci\u00E1lem vyu\u017Eit\u00ED ve fytoremedia\u010Dn\u00EDch technologi\u00EDch t\u011B\u017Ek\u00FDch kov\u016F" . . "Fabrik, Ivo" . "Stanoven\u00ED metalothioneinu u transgenn\u00EDch rostlin s potenci\u00E1lem vyu\u017Eit\u00ED ve fytoremedia\u010Dn\u00EDch technologi\u00EDch t\u011B\u017Ek\u00FDch kov\u016F" . "V t\u00E9to pr\u00E1ci jsme se zam\u011B\u0159ili na zkoum\u00E1n\u00ED transgenn\u00EDch rostlin exprimuj\u00EDc\u00EDch metalothionein. Jedna z \u00FAloh tohoto proteinu v \u017Eivo\u010Di\u0161n\u00E9m organismu je ochrana p\u0159ed toxick\u00FDmi koncentracemi t\u011B\u017Ek\u00FDch kov\u016F. U rostlin tuto funkci zast\u00E1vaj\u00ED fytochelatiny -- peptidy bohat\u00E9 na cystein. Spolu\u00FA\u010Dast obou mechanizm\u016F u modifikovan\u00FDch rostlin zvy\u0161uje jejich toleranci k t\u011B\u017Ek\u00FDm kov\u016Fm. Tato skute\u010Dnost m\u016F\u017Ee b\u00FDt vyu\u017Eita ve fytoremedia\u010Dn\u00EDch technologi\u00EDch, kde rostliny s vysokou akumula\u010Dn\u00ED schopnost\u00ED sni\u017Euj\u00ED n\u00E1klady a efektivitu dekontaminace. Stanovili jsme obsah transgenn\u00EDho metalothionein u r\u016Fzn\u00FDch klon\u016F tab\u00E1ku a porovnali ho z experiment\u00E1ln\u011B zji\u0161t\u011Bnou akumula\u010Dn\u00ED schopnost\u00ED rostlin. Z\u00E1rove\u0148 jsme stanovili obsah celkov\u00FDch thiol\u016F a obsah redukovan\u00E9ho a oxidovan\u00E9ho glutathionu, fytochelatinu 2 a cysteinu, dal\u0161\u00EDch slou\u010Denin pod\u00EDlej\u00EDc\u00EDch se na detoxikaci t\u011B\u017Ek\u00FDch kov\u016F. Prok\u00E1zali jsme v\u00FDraznou expresi transgenn\u00EDho metalothioneinu, kter\u00FD m\u011Bl nejv\u011Bt\u0161\u00ED pod\u00EDl na zji\u0161t\u011Bn\u00E9 vy\u0161\u0161\u00ED akumula\u010Dn\u00ED schopnost\u00ED transgenn\u00EDch rostlin tab\u00E1ku."@cs . "2008-01-01+01:00"^^ . "Havel, Ladislav" . "In the present work we aimed on investigation of transgenic plants expressed metallothionein. One of the key roles of this protein is protection of an animal organism against heavy metals. At plants cysteine rich peptides called phytochelatins play the key heavy metal detoxification role. Cooperation of both mechanisms can enhance the ability of plants to withstand heavy metal toxic effects. This feature can be employed in phytoremediation technologies, where the plants with high accumulative ability would decrease the costs and increase the effectiveness of decontamination process. In the present work we determined the content of transgenic metallothionein at various clones of tobacco and compared the results with ability of plants to accumulate heavy metals. In addition we determined total content of thiols and content of reduced and oxidized glutathione, phytochelatin2 and cysteine. We found marked expression of transgenic metallothionein, which had the great participation on higher accumulative ab"@en . . . "978-80-87011-18-8" . "397022" . . "Vliv abiotick\u00FDch a biotick\u00FDch stresor\u016F na vlastnosti rostlin" . . "\u010Cesk\u00E1 zem\u011Bd\u011Blsk\u00E1 univerzita v Praze" . "6"^^ . "Shestivska, Violetta" . . "Stanoven\u00ED metalothioneinu u transgenn\u00EDch rostlin s potenci\u00E1lem vyu\u017Eit\u00ED ve fytoremedia\u010Dn\u00EDch technologi\u00EDch t\u011B\u017Ek\u00FDch kov\u016F"@cs . "Praha, \u010Cesk\u00E1 republika" . "9"^^ . "Determination of metallothionein at transgenic plants - Using for heavy metal phytoremediation technologies"@en . "Mackova, Martina" .