. . "Measuring NO production by plant tissues and suspension cultured cells" . . . "We describe an inexpensive and reliable detector for measuring NO emitted in the gas phase from plants. The method relies on the use of a strong oxidizer to convert NO to NO2 and subsequent capture of NO2 by a Griess reagent trap. The set-up approaches the sensitivity for NO comparable to that of instruments based on chemiluminescence and photoacoustic detectors. We demonstrate the utility of our set-up by measuring NO produced by a variety of well-established plant sources. NO produced by nitrate reductase (NR) in tobacco leaves and barley aleurone was readily detected as was the production of NO from nitrite by the incubation medium of barley aleurone. Arabidopsis mutants that overproduce NO or lack NO-synthase (AtNOS1) also displayed the expected NO synthesis phenotype when assayed by our set-up. We could also measure NO production from elicitor-treated suspension cultured cells using this set-up. Further, we have focused on detection of NO by a widely used fluorescent probe 4-amino-5-methylamino-2"@en . "Rein\u00F6hl, Vil\u00E9m" . "270;284" . "15"^^ . "RIV/62156489:43210/08:00117842" . "M\u011B\u0159en\u00ED produkce NO rostlinn\u00FDmi pletivy a bun\u011B\u010Dn\u00FDmi suspenzn\u00EDmi kulturami"@cs . "M\u011B\u0159en\u00ED produkce NO rostlinn\u00FDmi pletivy a bun\u011B\u010Dn\u00FDmi suspenzn\u00EDmi kulturami"@cs . "Jones, Russell L." . "2" . . "Measuring NO production by plant tissues and suspension cultured cells"@en . "43210" . "nitric oxide; oxidizer column; Griess reagent"@en . . "2"^^ . . "3"^^ . "Molecular Plant" . . . "1" . . "Measuring NO production by plant tissues and suspension cultured cells" . . . "V\u00EDte\u010Dek, Jan" . "Measuring NO production by plant tissues and suspension cultured cells"@en . . . . "We describe an inexpensive and reliable detector for measuring NO emitted in the gas phase from plants. The method relies on the use of a strong oxidizer to convert NO to NO2 and subsequent capture of NO2 by a Griess reagent trap. The set-up approaches the sensitivity for NO comparable to that of instruments based on chemiluminescence and photoacoustic detectors. We demonstrate the utility of our set-up by measuring NO produced by a variety of well-established plant sources. NO produced by nitrate reductase (NR) in tobacco leaves and barley aleurone was readily detected as was the production of NO from nitrite by the incubation medium of barley aleurone. Arabidopsis mutants that overproduce NO or lack NO-synthase (AtNOS1) also displayed the expected NO synthesis phenotype when assayed by our set-up. We could also measure NO production from elicitor-treated suspension cultured cells using this set-up. Further, we have focused on detection of NO by a widely used fluorescent probe 4-amino-5-methylamino-2" . . "Popisujeme nen\u00E1ro\u010Dn\u00FD a spolehliv\u00FD detektor pro m\u011B\u0159en\u00ED NO emitovan\u00E9ho rostlinami do plynn\u00E9 f\u00E1ze."@cs . "[AC3D967B4AFF]" . "RIV/62156489:43210/08:00117842!RIV08-MSM-43210___" . "378205" . "1674-2052" . "P(1P05ME757)" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" .