"Brzobohat\u00FD, B\u0159etislav" . "421371" . "RIV/62156489:43210/07:00117773" . "Characterization of the AHP gene family using expression profiling and cytokinin analysis"@en . . "Brno" . . "978-80-7375-090-9" . "2"^^ . . "U vy\u0161\u0161\u00EDch rostlin se v\u00EDcestup\u0148ov\u00FD dvoukomponentn\u00ED syst\u00E9m \u00FA\u010Dastn\u00ED p\u0159enosu sign\u00E1lu n\u011Bkter\u00FDch hormon\u016F a reakc\u00ED na stres nebo zm\u011Bnu sv\u011Bteln\u00FDch podm\u00EDnek. Faktory s p\u0159ena\u0161e\u010Dovou dom\u00E9nou (AHP) transportuj\u00ED fosf\u00E1tovou skupinu z membr\u00E1nov\u00FDch receptor\u016F na regul\u00E1tory odpov\u011Bdi. Ke kvantifikaci ust\u00E1len\u00FDch hladin transkript\u016F jednotliv\u00FDch AHP gen\u016F byla pou\u017Eita metoda RT-PCR v re\u00E1ln\u00E9m \u010Dase. Testov\u00E1ny byly vzorky poch\u00E1zej\u00EDc\u00ED z list\u016F, ko\u0159en\u016F, stonk\u016F, kv\u011Bt\u016F a \u0161e\u0161ul\u00ED Arabidopsis thaliana. Tut\u00E9\u017E metodu jsme pou\u017Eili ke sledov\u00E1n\u00ED vlivu cytokinin\u016F (CK) na expresi t\u00E9to genov\u00E9 rodiny v semen\u00E1\u010Dc\u00EDch. Byl srovn\u00E1v\u00E1n rozd\u00EDl v p\u016Fsoben\u00ED exogenn\u011B aplikovan\u00FDch aromatick\u00FDch CK a CK izoprenoidn\u00EDho typu produkovan\u00FDch enzymem izopentyltransfer\u00E1zou (ipt). Po aktivaci exprese ipt jsme zaznamenali v\u00FDrazn\u00FD n\u00E1r\u016Fst deriv\u00E1t\u016F trans-zeatinu a pouze minim\u00E1ln\u00ED zm\u011Bny v mno\u017Estv\u00ED ostatn\u00EDch izoprenoidn\u00EDch CK. K p\u0159echodn\u00E9mu n\u00E1r\u016Fstu exprese gen\u016F AHP1-4 do\u0161lo po kr\u00E1tkodob\u00E9m p\u016Fsoben\u00ED jak aromatick\u00FDch tak i izoprenoidn\u00EDch CK. Pokud v\u0161ak byly semen\u00E1\u010Dky kultivo" . "Expresn\u00ED profil AHP gen\u016F a stanoven\u00ED hladiny cytokinin\u016F u Arabidopsis thaliana" . "Expresn\u00ED profil AHP gen\u016F a stanoven\u00ED hladiny cytokinin\u016F u Arabidopsis thaliana"@cs . "[C7D83D7EE601]" . "P(LC06034)" . "3"^^ . . "Malbeck, Ji\u0159\u00ED" . "U vy\u0161\u0161\u00EDch rostlin se v\u00EDcestup\u0148ov\u00FD dvoukomponentn\u00ED syst\u00E9m \u00FA\u010Dastn\u00ED p\u0159enosu sign\u00E1lu n\u011Bkter\u00FDch hormon\u016F a reakc\u00ED na stres nebo zm\u011Bnu sv\u011Bteln\u00FDch podm\u00EDnek. Faktory s p\u0159ena\u0161e\u010Dovou dom\u00E9nou (AHP) transportuj\u00ED fosf\u00E1tovou skupinu z membr\u00E1nov\u00FDch receptor\u016F na regul\u00E1tory odpov\u011Bdi. Ke kvantifikaci ust\u00E1len\u00FDch hladin transkript\u016F jednotliv\u00FDch AHP gen\u016F byla pou\u017Eita metoda RT-PCR v re\u00E1ln\u00E9m \u010Dase. Testov\u00E1ny byly vzorky poch\u00E1zej\u00EDc\u00ED z list\u016F, ko\u0159en\u016F, stonk\u016F, kv\u011Bt\u016F a \u0161e\u0161ul\u00ED Arabidopsis thaliana. Tut\u00E9\u017E metodu jsme pou\u017Eili ke sledov\u00E1n\u00ED vlivu cytokinin\u016F (CK) na expresi t\u00E9to genov\u00E9 rodiny v semen\u00E1\u010Dc\u00EDch. Byl srovn\u00E1v\u00E1n rozd\u00EDl v p\u016Fsoben\u00ED exogenn\u011B aplikovan\u00FDch aromatick\u00FDch CK a CK izoprenoidn\u00EDho typu produkovan\u00FDch enzymem izopentyltransfer\u00E1zou (ipt). Po aktivaci exprese ipt jsme zaznamenali v\u00FDrazn\u00FD n\u00E1r\u016Fst deriv\u00E1t\u016F trans-zeatinu a pouze minim\u00E1ln\u00ED zm\u011Bny v mno\u017Estv\u00ED ostatn\u00EDch izoprenoidn\u00EDch CK. K p\u0159echodn\u00E9mu n\u00E1r\u016Fstu exprese gen\u016F AHP1-4 do\u0161lo po kr\u00E1tkodob\u00E9m p\u016Fsoben\u00ED jak aromatick\u00FDch tak i izoprenoidn\u00EDch CK. Pokud v\u0161ak byly semen\u00E1\u010Dky kultivo"@cs . "5;5" . "gene expression"@en . . "Characterization of the AHP gene family using expression profiling and cytokinin analysis"@en . . "Hradilov\u00E1, Jana" . . "Funk\u010Dn\u00ED genomika a proteomika ve \u0161lecht\u011Bn\u00ED rostlin" . "Expresn\u00ED profil AHP gen\u016F a stanoven\u00ED hladiny cytokinin\u016F u Arabidopsis thaliana" . "43210" . "Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B" . . . . . . "RIV/62156489:43210/07:00117773!RIV08-MSM-43210___" . "In higher plants, histidine-aspartate phosphorelays are involved in hormone, stress and light signaling via two-component systems of signal transduction. Histidine-containing phosphotransmitters (AHPs) transfer a phosphoryl group from membrane receptors to effectors in the nucleus. Five AHPs have been identified in Arabidopsis thaliana. Real-time RT-PCR was employed to quantify steady state levels of individual transcripts in leaves, roots, stems, flowers and siliques. The transcripts of all AHPs were detected in every organ examined, although at different levels. High organ specificity of gene expression was found in AHP1, AHP2, AHP4 while expression of AHP3 and AHP5 appears more ubiquitous. We also analyzed the regulation by cytokinins of AHP gene expression. We compared the effects on steady state levels of AHP transcripts of a short-term treatment with an aromatic cytokinin and increase in endogenous isoprenoid cytokinin levels using an activable ipt system in 8-day-old Arabidopsis seedlings. Foll"@en . . . "Expresn\u00ED profil AHP gen\u016F a stanoven\u00ED hladiny cytokinin\u016F u Arabidopsis thaliana"@cs . "1"^^ .