. . . . "B\u00EDlek, Karel" . . "Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds" . "VIIth International conference of PhD. and MSc. students %22Genetics and Animal Breeding%22" . . . . "RIV/62156489:43210/07:00107957!RIV08-GA0-43210___" . "P(GA523/06/1302), P(GD523/03/H076)" . . "Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds"@en . . . "This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression level was found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provide a new knowled"@en . "Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds"@cs . . "6"^^ . "Brno" . "Knoll, Ale\u0161" . "Brno" . "Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds"@cs . "2007-05-17+02:00"^^ . . . . "Svobodov\u00E1, Kate\u0159ina" . "This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression level was found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provide a new knowled"@cs . "RIV/62156489:43210/07:00107957" . "3"^^ . "78;83" . "414376" . "This research was focused on investigation of gene expression of selected genes via the real-time quantitative PCR. Specific primers for CDK4 and TP53BP1 were designed on the basis of the sequences clones obtained from the subtractive hybridization and gene expression was analysed. Two groups of samples isolated skeletal muscle-specific RNA were compared, the first one was obtained from the Pietrain adult sows and their fetuses, the second one was obtained from Large White, respectively. The stability values of candidate reference genes (ACTB, EEF1A1, EEF2, GAPDH, H3F, HPRT1, PPIA and TOP2B) were made for both breeds. The expression levels of the investigated genes were varying by adult sows used as control samples. The higher or lower expression level was found out in few samples; one of the samples of gene CDK4 was expressed near to 0.001-fold, and two of samples of gene TP53BP1 were expressed higher then 18-fold, 52-fold respectively. The long-range goal of this research is to provide a new knowled" . "Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds" . . . "978-80-7375-053-4" . "[D43E0A0D0276]" . "3"^^ . . "43210" . . "Mendelova zem\u011Bd\u011Blsk\u00E1 a lesnick\u00E1 univerzita v Brn\u011B. Edi\u010Dn\u00ED st\u0159edisko" . "real time PCR; gene expression; skeletal muscle"@en . "Comparison of prenatal muscle tissue expression profiles of genes CDK4 and TP53BP1 in two pig breeds"@en .