. "424078" . "HPLC/ESI-MS stanoven\u00ED sanguinarinu a chelerythrinu po extrakci superkritick\u00FDm oxidem uhli\u010Dit\u00FDm zs spajkkovan\u00FDch a re\u00E1ln\u00FDch vzork\u016F plasmy."@cs . . "Dva pol\u00E1rn\u00ED benzo[c]fenanthridinov\u00E9 alkaloidy, chelerythrin (CHE) a dihydrochelerythrin (DHCHE), byly extrahov\u00E1ny p\u0159i 35 \u00B0C a 10 MPa (15 MPa u re\u00E1ln\u00FDch vzork\u016F) z re\u00E1ln\u00FDch a spajkovan\u00FDch vzork\u016F plasmy s p\u0159ijateln\u00FDmi n\u00E1vratnostmi (95.1 % and 81.0 %) pomoc\u00ED superkritick\u00E9ho oxidu uhli\u010Dit\u00E9ho modifikovan\u00E9ho sm\u011Bs\u00ED (1:1, v/v) voda/methanol. Alkaloidy byly stanoveny HPLC/ESI-MS na kolon\u011B Zorbax SB-CN gradientovou eluc\u00ED mobiln\u00ED f\u00E1zi methanol/50 mM mraven\u010Dan amonn\u00FD. LOD byl 1.22 ng (3.50 pmol) pro CHE a 0.95 ng (2.72 pmol) pro DHCHE v 1 ml vzorku. Pr\u016Fm\u011Brn\u00E1 n\u00E1vratnost byla 100.1 % pro spajkovan\u00FD i re\u00E1ln\u00FD vzorek extrakt\u016F plasmy. Metodou byly extrahov\u00E1ny a analyzov\u00E1ny re\u00E1ln\u00E9 vzorky plasmy potkan\u016F."@cs . "Two polar benzo[c]phenanthridine alkaloids, chelerythrine (CHE) and dihydrochelerythrine (DHCHE), were extracted at 35 \u00B0C and 10 MPa (15 MPa for real samples) from real and spiked plasma samples with acceptable recoveries (95.1 % and 81.0 %, respectively) using near-critical CO2 modified with aqueous (1:1, v/v) methanol. The alkaloids were quantified by a liquid chromatographic/electrospray mass spectrometric (LC/ESI-MS) method on a Zorbax SB-CN column (75 x 4.6 mm, 3.5 mm particle size) using methanol (organic phase) and 50 mM ammonium formiate (aqueous phase) as a mobile phase. A linear gradient 0 to 1 min - isocratic at 60% aqueous phase (v/v); from 1.0 to 7.0 min - 60% to 71% aqueous phase (v/v); from 7.0 to 18.0 min - 71% to 60% aqueous phase (v/v) was applied. The limit of detection was 1.22 ng (3.50 pmol) for CHE and 0.95 ng (2.72 pmol) for DHCHE per 1 ml of the sample. The linearity of the calibration curves was satisfactory as indicated by coefficients of determination 0.9979 and 0.9995 for C"@en . "9"^^ . . . . . "P(GA525/07/0871)" . "RIV/62156489:43210/07:00099035!RIV08-GA0-43210___" . "High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples" . "1348;1356" . "43210" . . . "[0AB513B48528]" . "HPLC/ESI-MS stanoven\u00ED sanguinarinu a chelerythrinu po extrakci superkritick\u00FDm oxidem uhli\u010Dit\u00FDm zs spajkkovan\u00FDch a re\u00E1ln\u00FDch vzork\u016F plasmy."@cs . "HPLC; alkaloids; plasma samples"@en . "Kub\u00E1\u0148, Vlastimil" . "0039-9140" . "Ulrichov\u00E1, Jitka" . . . . "6"^^ . . "RIV/62156489:43210/07:00099035" . "High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples"@en . . "Lojkov\u00E1, Lea" . . "High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples"@en . . . "Klejdus, Bo\u0159ivoj" . "3"^^ . "High Performance Liquid Chromatographic-Electrospray Mass Spectrometric Quantification (HPLC/ESI-MS) of Sanguinarine and Chelerythrine in Near Supercritical Carbon Dioxide Extracts from Spiked and Real Plasma Samples" . "Talanta" . "\u0160im\u00E1nek, Vil\u00EDm" . "4" . . "Two polar benzo[c]phenanthridine alkaloids, chelerythrine (CHE) and dihydrochelerythrine (DHCHE), were extracted at 35 \u00B0C and 10 MPa (15 MPa for real samples) from real and spiked plasma samples with acceptable recoveries (95.1 % and 81.0 %, respectively) using near-critical CO2 modified with aqueous (1:1, v/v) methanol. The alkaloids were quantified by a liquid chromatographic/electrospray mass spectrometric (LC/ESI-MS) method on a Zorbax SB-CN column (75 x 4.6 mm, 3.5 mm particle size) using methanol (organic phase) and 50 mM ammonium formiate (aqueous phase) as a mobile phase. A linear gradient 0 to 1 min - isocratic at 60% aqueous phase (v/v); from 1.0 to 7.0 min - 60% to 71% aqueous phase (v/v); from 7.0 to 18.0 min - 71% to 60% aqueous phase (v/v) was applied. The limit of detection was 1.22 ng (3.50 pmol) for CHE and 0.95 ng (2.72 pmol) for DHCHE per 1 ml of the sample. The linearity of the calibration curves was satisfactory as indicated by coefficients of determination 0.9979 and 0.9995 for C" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Kosina, Pavel" . "72" .