"8" . . "The process of resistance to platinum cytostatic formation"@en . . . "Salzman, Richard" . "Binkov\u00E1, Hana" . "This work is aimed to study of metallothionein role in formation of the resistance to antitumour therapeutics, especially based on platinum. Tumour cell lines resistant and sensitive to cisplatim were used. Single cell lines were characterized in the term of chromozomal aberations. In this work the cell lines were characterized by determination of intracellular esterase activity. In the experiment the possibility to determine the number of living cells in neuroblastoma cell lines was tested. In sensitive cell the esterase activity was markedly higher than in resistant one. The detected intracellular esterases activity was directly proportional to cell number. The detection limit was about 600 cells in sensitive and 1000 in resistant cell lines. Then the change of metallothionein level was studied by our proposed electroanalytical method. The cisplatin level was higher in resistant than in sensitive cell lines. Based on our previously published results the interaction between platinum therapeutic and m"@en . "43210" . . . "[FB8955B83F45]" . "Proces vytv\u00E1\u0159en\u00ED rezistence v\u016F\u010Di platinov\u00FDm cytostatik\u016Fm" . "CZ - \u010Cesk\u00E1 republika" . "Horakov\u00E1, Zuzana" . . "Proces vytv\u00E1\u0159en\u00ED rezistence v\u016F\u010Di platinov\u00FDm cytostatik\u016Fm"@cs . "RIV/62156489:43210/06:00103470" . "cisplatin; metallothionein"@en . "Adam, Vojt\u011Bch" . "0009-2770" . "Na\u0161e pr\u00E1ce se zam\u011B\u0159uje na studium \u00FAlohy metalothioneinu v procesu vzniku rezistence v\u016F\u010Di protin\u00E1dorov\u00FDm l\u00E9\u010Div\u016Fm p\u0159edev\u0161\u00EDm na b\u00E1zi platiny. Byly pou\u017Eity bun\u011B\u010Dn\u00E9 linie rezistentn\u00ED a sensitivn\u00EDch k cisplatin\u011B. Jednotliv\u00E9 bun\u011B\u010Dn\u00E9 linie charakterizov\u00E1ny z hlediska chromozom\u00E1ln\u00EDch aberac\u00ED. Nyn\u00ED jsme charakterizovali bun\u011B\u010Dn\u00E9 linie v z\u00E1vislosti na aktivit\u011B intracelul\u00E1rn\u00EDch ester\u00E1s. V experimentu byla testov\u00E1na mo\u017Enost uskute\u010Dnit detekci po\u010Dtu \u017Eiv\u00FDch bun\u011Bk ve vzorc\u00EDch neuroblastomov\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED. Na z\u00E1klad\u011B zn\u00E1m\u00E9ho po\u010Dtu bun\u011Bk, kter\u00E9 byly homogenizov\u00E1ny v dan\u00E9m objemu, byly do reak\u010Dn\u00ED sm\u011Bsi pro stanoven\u00ED intracelul\u00E1rn\u00EDch esteras vn\u00E1\u0161eny ekvivalenty po\u010Dtu bun\u011Bk v rozmez\u00ED 0 a\u017E 7000. U senzitivn\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED byla aktivita esteras v\u00FDrazn\u011B vy\u0161\u0161\u00ED v porovn\u00E1n\u00ED s kulturou rezistentn\u00ED bun\u011B\u010Dn\u00E9 linie. Detekovan\u00E1 aktivita intracelul\u00E1rn\u00EDch esteras byla p\u0159\u00EDmo \u00FAm\u011Brn\u00E1 po\u010Dtu bun\u011Bk a limit detekce se pohyboval asi kolem 600 bun\u011Bk pro senzitivn\u00ED neuroblastomov\u00E9 kultury a asi kolem 1000 bun\u011Bk pro rezistentn\u00ED bun\u011B\u010Dn\u00E9 ku" . . . "Kizek, Ren\u00E9" . "3"^^ . "RIV/62156489:43210/06:00103470!RIV07-GA0-43210___" . . . "Proces vytv\u00E1\u0159en\u00ED rezistence v\u016F\u010Di platinov\u00FDm cytostatik\u016Fm"@cs . "Svoboda, Michal" . "Proces vytv\u00E1\u0159en\u00ED rezistence v\u016F\u010Di platinov\u00FDm cytostatik\u016Fm" . . "581;582" . "Na\u0161e pr\u00E1ce se zam\u011B\u0159uje na studium \u00FAlohy metalothioneinu v procesu vzniku rezistence v\u016F\u010Di protin\u00E1dorov\u00FDm l\u00E9\u010Div\u016Fm p\u0159edev\u0161\u00EDm na b\u00E1zi platiny. Byly pou\u017Eity bun\u011B\u010Dn\u00E9 linie rezistentn\u00ED a sensitivn\u00EDch k cisplatin\u011B. Jednotliv\u00E9 bun\u011B\u010Dn\u00E9 linie charakterizov\u00E1ny z hlediska chromozom\u00E1ln\u00EDch aberac\u00ED. Nyn\u00ED jsme charakterizovali bun\u011B\u010Dn\u00E9 linie v z\u00E1vislosti na aktivit\u011B intracelul\u00E1rn\u00EDch ester\u00E1s. V experimentu byla testov\u00E1na mo\u017Enost uskute\u010Dnit detekci po\u010Dtu \u017Eiv\u00FDch bun\u011Bk ve vzorc\u00EDch neuroblastomov\u00FDch bun\u011B\u010Dn\u00FDch lini\u00ED. Na z\u00E1klad\u011B zn\u00E1m\u00E9ho po\u010Dtu bun\u011Bk, kter\u00E9 byly homogenizov\u00E1ny v dan\u00E9m objemu, byly do reak\u010Dn\u00ED sm\u011Bsi pro stanoven\u00ED intracelul\u00E1rn\u00EDch esteras vn\u00E1\u0161eny ekvivalenty po\u010Dtu bun\u011Bk v rozmez\u00ED 0 a\u017E 7000. U senzitivn\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED byla aktivita esteras v\u00FDrazn\u011B vy\u0161\u0161\u00ED v porovn\u00E1n\u00ED s kulturou rezistentn\u00ED bun\u011B\u010Dn\u00E9 linie. Detekovan\u00E1 aktivita intracelul\u00E1rn\u00EDch esteras byla p\u0159\u00EDmo \u00FAm\u011Brn\u00E1 po\u010Dtu bun\u011Bk a limit detekce se pohyboval asi kolem 600 bun\u011Bk pro senzitivn\u00ED neuroblastomov\u00E9 kultury a asi kolem 1000 bun\u011Bk pro rezistentn\u00ED bun\u011B\u010Dn\u00E9 ku"@cs . "100" . "The process of resistance to platinum cytostatic formation"@en . . "Bla\u0161t\u00EDk, Ond\u0159ej" . "495107" . "7"^^ . . "Chemick\u00E9 listy" . "2"^^ . "P(GP525/04/P132)" . . . .