"Increase in content of metallothionein as marker of resistance to cisplatin treatment"@en . "Increase in content of metallothionein as marker of resistance to cisplatin treatment" . . "43210" . "479173" . . . . . "Byli pops\u00E1ny 4 t\u0159\u00EDdy metalothioneinu. Lidsk\u00FD metalothionein pat\u0159\u00ED do prvn\u00ED skupiny a jsou k\u00F3dov\u00E1ny rodinou s deseti isofomami. Pro jejich schopnosti vytv\u00E1\u0159et komplexy s kovy jako je zinek, kadmium apod. m\u00E1 d\u016Fle\u017Eitou fyziologickou funkci v regulaci hladin t\u011Bchto iontu v organizmu. Je pravd\u011Bpodobn\u00E9, \u017Ee sehr\u00E1vaj\u00ED \u00FAlohu v n\u00E1dorov\u00E9 rezistenci p\u0159i l\u00E9\u010Db\u011B platinov\u00FDmi cytostatiky. V t\u00E9to pr\u00E1ci jsme zkoumali interakci platinov\u00FDch cytostatik s MT a koncentraci MT u n\u00E1dorov\u00FDch lini\u00EDch. My jsme pou\u017Eily bun\u011B\u010Dn\u00E9 linie z neuroblastomu a to linie rezistentn\u00ED (UKF-NB 2 CDDP, UKF-NB 3 CDDP and UKF-NB 4 CDDP) a senzitivn\u00ED (UKF-NB 2, UKF-NB 3 and UKF-NB 4). P\u0159\u00EDtomnost cisplatiny u bun\u011B\u010Dn\u00FDch lini\u00ED spust\u00ED tvorbu thiolov\u00FDch l\u00E1tek, jejich mno\u017Estv\u00ED vzrostlo a\u017E o 1000% v porovn\u00E1n\u00ED s kontrolou.."@cs . "V\u00FDznam zv\u00FD\u0161en\u00ED obsahu metalothioneinu v rezistenci k l\u00E9\u010Db\u011B cisplatinou"@cs . . "Eckschlager, Tom\u00E1\u0161" . "Adam, Vojt\u011Bch" . "Kizek, Ren\u00E9" . "Z\u00EDtka, Ond\u0159ej" . . . "[8AAABDE1D749]" . "Bla\u0161t\u00EDk, Ond\u0159ej" . "Beklov\u00E1, Miroslava" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "V\u00FDznam zv\u00FD\u0161en\u00ED obsahu metalothioneinu v rezistenci k l\u00E9\u010Db\u011B cisplatinou"@cs . "Increase in content of metallothionein as marker of resistance to cisplatin treatment"@en . "RIV/62156489:43210/06:00103447" . . "4"^^ . "Svoboda, Michal" . . . "Four classes of metallothioneins (MT1, MT2, MT3 and MT4) have been described until now. Human metallothioneins belongs to I. class of metallothioneins (MT1) and are encoded by gene family with 10 isoforms. Due to their high affinity to heavy metals e.g. zinc, copper and/or cadmium, homeostatic control and detoxification of the metals are their main physiological function at evolutionary different animal organisms. Moreover, it has been discussed that expression of MT could relate with resistance to a treatment by anticancer drugs based on heavy metals, most of all, platinum group metals. The aim of this work was to investigate interactions of MT with cisplatin and to determine a content of MT at tumour cell lines. We used cell lines obtained from neuroblastome that were cisplatin sensitive (UKF-NB 2, UKF-NB 3 and UKF-NB 4) and/or cisplatin resistant (UKF-NB 2 CDDP, UKF-NB 3 CDDP and UKF-NB 4 CDDP). The samples were analysed with AUTOLAB Analyser (EcoChemie, Netherlands). The supporting electrolyte c" . "Clinical chemistry" . "RIV/62156489:43210/06:00103447!RIV07-GA0-43210___" . "2"^^ . . "6" . . . . "9"^^ . "P(GP525/04/P132)" . "Four classes of metallothioneins (MT1, MT2, MT3 and MT4) have been described until now. Human metallothioneins belongs to I. class of metallothioneins (MT1) and are encoded by gene family with 10 isoforms. Due to their high affinity to heavy metals e.g. zinc, copper and/or cadmium, homeostatic control and detoxification of the metals are their main physiological function at evolutionary different animal organisms. Moreover, it has been discussed that expression of MT could relate with resistance to a treatment by anticancer drugs based on heavy metals, most of all, platinum group metals. The aim of this work was to investigate interactions of MT with cisplatin and to determine a content of MT at tumour cell lines. We used cell lines obtained from neuroblastome that were cisplatin sensitive (UKF-NB 2, UKF-NB 3 and UKF-NB 4) and/or cisplatin resistant (UKF-NB 2 CDDP, UKF-NB 3 CDDP and UKF-NB 4 CDDP). The samples were analysed with AUTOLAB Analyser (EcoChemie, Netherlands). The supporting electrolyte c"@en . . . "cancer; cisplatin; metallothionein"@en . "52" . "Increase in content of metallothionein as marker of resistance to cisplatin treatment" . "Pr\u016F\u0161a, Richard" . "A174;A175" . . "0009-9147" .