"2"^^ . . "6"^^ . . "Molekul\u00E1rn\u00ED markery genetick\u00E9 variability u odr\u016Fd tritikale registrovan\u00FDch v \u010Cesk\u00E9 republice"@cs . . . "Vyhn\u00E1nek, Tom\u00E1\u0161" . "RIV/62156489:43210/06:00099021!RIV07-GA0-43210___" . "Molecular markers of genetic variability in triticale varieties registered in the Czech Republic" . "RIV/62156489:43210/06:00099021" . "5" . "LIV" . "Molecular markers of genetic variability in triticale varieties registered in the Czech Republic" . . "P(GP521/03/P173)" . "CZ - \u010Cesk\u00E1 republika" . "1211-8516" . "Molecular markers of genetic variability in triticale varieties registered in the Czech Republic"@en . "Genetic variability was detected in 15 varieties of triticale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR) registered in the Czech Republic by means of polymorphism of DNA using the RAPD method and the SSR method. For detection we used 80 RAPD primers. The lower reproducibility of the RAPD markers was resolved by means of repeated analyses (3--4 times). On the basis of statistical evaluation a dendrogram was set up, which allows highly significantly to differentiate the varieties Kolor, Modus and Tornado. The remaining 12 analysed varieties formed 4 clusters. In addition to the RAPD markers a protocol of detection of DNA polymorphism was elaborated and optimised with microsatellite (SSR) markers. For the analyses we used 2 SSR markers (1A chromosome [Xpsp2999] and 1B chromosome [Xpsp3000]), which have been discovered in wheat (T. aestivum L.). Basing on these two SSR markers the only variety Triamant was distinguished from the clusters of the other analysed varieties." . . . "Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis" . . . . "Molekul\u00E1rn\u00ED markery genetick\u00E9 variability u odr\u016Fd tritikale registrovan\u00FDch v \u010Cesk\u00E9 republice"@cs . . . . "Genetic variability was detected in 15 varieties of triticale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR) registered in the Czech Republic by means of polymorphism of DNA using the RAPD method and the SSR method. For detection we used 80 RAPD primers. The lower reproducibility of the RAPD markers was resolved by means of repeated analyses (3--4 times). On the basis of statistical evaluation a dendrogram was set up, which allows highly significantly to differentiate the varieties Kolor, Modus and Tornado. The remaining 12 analysed varieties formed 4 clusters. In addition to the RAPD markers a protocol of detection of DNA polymorphism was elaborated and optimised with microsatellite (SSR) markers. For the analyses we used 2 SSR markers (1A chromosome [Xpsp2999] and 1B chromosome [Xpsp3000]), which have been discovered in wheat (T. aestivum L.). Basing on these two SSR markers the only variety Triamant was distinguished from the clusters of the other analysed varieties."@en . "Molecular markers of genetic variability in triticale varieties registered in the Czech Republic"@en . "[892B2CF2C9CF]" . "2"^^ . . "Genetick\u00E1 variabilita byla detekov\u00E1na metodami molekul\u00E1rn\u00ED genetiky (RAPD a SSR) u 15 odr\u016Fd tritikale (XTriticosecale Wittmack., 2n = 6x = 42, BBAARR), kter\u00E9 jsou registrov\u00E1ny v \u010Cesk\u00E9 republice. Pro detekci polymorfizmu DNA bylo vyu\u017Eito 80 RAPD primer\u016F. Ni\u017E\u0161\u00ED reprodukovatelnost RAPD anal\u00FDz byla kompenzov\u00E1na opakov\u00E1n\u00EDm anal\u00FDz (3--4x). Na z\u00E1klad\u011B statistick\u00E9ho vyhodnocen\u00ED byl sestaven dendrogram, kde je mo\u017Eno vysoce pr\u016Fkazn\u011B odli\u0161it odr\u016Fdy Kolor, Modus a Tornado. Zb\u00FDvaj\u00EDc\u00EDch 12 analyzovan\u00FDch odr\u016Fd tvo\u0159\u00ED \u010Dty\u0159i klastery. Byl optimalizov\u00E1n protokol detekce variability DNA pomoc\u00ED mikrosatelitn\u00EDch marker\u016F u tritikale. Pro anal\u00FDzy byly vyu\u017Eity dva SSR markery (1A chromozom [Xpsp2999] a 1B chromozom [Xpsp3000]), kter\u00E9 byly pops\u00E1ny u p\u0161enice obecn\u00E9 (T. aestivum L.). Na z\u00E1klad\u011B pou\u017Eit\u00FDch dvou SSR marker\u016F se poda\u0159ilo odli\u0161it odr\u016Fdu Triamant od klaster\u016F ostatn\u00EDch analyzovan\u00FDch odr\u016Fd."@cs . . "486619" . "149;154" . "RAPD; SSR; triticale"@en . "43210" . . "Bedn\u00E1\u0159, Jan" .