"Establishing of polar auxin transport in pea axillary buds released from apical dominance"@en . "Praha" . "43210" . . . "Ustaven\u00ED pol\u00E1rn\u00EDho transportu auxinu v axil\u00E1rn\u00EDch pupenech hrachu uvoln\u011Bn\u00FDch z apik\u00E1ln\u00ED dominance"@cs . . . "1"^^ . "RIV/62156489:43210/05:00084749" . "Kalousek, Petr" . . . . . . . . "PIN1; axillary buds; polar auxin transport"@en . "Apical dominance is a dominance of the shoot apex over the axillary buds. It is well-known that auxin accumulated in the apex is transported towards the roots by polar transport. The mechanism, whereby polar auxin transport in the shoot regulates outgrowth of axillary buds, is unknown. One of the possibilities is that shoot auxin transport inhibits polarization of the cells in axillary buds, disabling the export of auxin from the inhibited buds. We studied the expression patterns of genes involved in auxin transport and bud dormancy (AUX 1, PIN 1 and PsDRM1) and localization of the putative PsPIN1 protein using anti-AtPIN1 antibodies in axillary buds during release from apical dominance. Semi-quantitative RT-PCR revealed a decrease of PsDRM1 expression after decapitation or BA application to the axillary buds of intact plants. Expression of PsPIN1 and PsAUX1 increases already 2 hours after decapitation with a maximum of about 150% at 6 hours and also the application of BA dramatically increases expre" . "4"^^ . . "Proch\u00E1zka, Stanislav" . . . "S22;S22" . . . "Prague 2005" . "0006-3134" . "Establishing of polar auxin transport in pea axillary buds released from apical dominance" . "2005-07-07+02:00"^^ . "P(GP522/02/D137)" . . "Friml, Ji\u0159\u00ED" . "Ustaven\u00ED pol\u00E1rn\u00EDho transportu auxinu v axil\u00E1rn\u00EDch pupenech hrachu uvoln\u011Bn\u00FDch z apik\u00E1ln\u00ED dominance"@cs . "520514" . "5"^^ . "Establishing of polar auxin transport in pea axillary buds released from apical dominance" . "Apik\u00E1ln\u00ED dominance je dominance lody\u017En\u00EDho vrcholu nad axil\u00E1rn\u00EDmi pupeny."@cs . "RIV/62156489:43210/05:00084749!RIV06-GA0-43210___" . "\u00DAstav experiment\u00E1ln\u00ED botaniky AV \u010CR" . "[D643EE8982D1]" . "Establishing of polar auxin transport in pea axillary buds released from apical dominance"@en . "Balla, Jozef" . "Apical dominance is a dominance of the shoot apex over the axillary buds. It is well-known that auxin accumulated in the apex is transported towards the roots by polar transport. The mechanism, whereby polar auxin transport in the shoot regulates outgrowth of axillary buds, is unknown. One of the possibilities is that shoot auxin transport inhibits polarization of the cells in axillary buds, disabling the export of auxin from the inhibited buds. We studied the expression patterns of genes involved in auxin transport and bud dormancy (AUX 1, PIN 1 and PsDRM1) and localization of the putative PsPIN1 protein using anti-AtPIN1 antibodies in axillary buds during release from apical dominance. Semi-quantitative RT-PCR revealed a decrease of PsDRM1 expression after decapitation or BA application to the axillary buds of intact plants. Expression of PsPIN1 and PsAUX1 increases already 2 hours after decapitation with a maximum of about 150% at 6 hours and also the application of BA dramatically increases expre"@en . . "Biologia Plantarum, 2nd International Symposium %22Auxins and Cytokinins in Plant Development%22, Book of Abstracts" . . "Rein\u00F6hl, Vil\u00E9m" .