"Somatic embryogenesis from zygotic embryos of Schisandra chinensis"@cs . "RIV/62156489:43210/05:00004744!RIV06-GA0-43210___" . . . . "Somatic embryogenesis from zygotic embryos of Schisandra chinensis"@en . "4"^^ . "abscisic acied; maturation; medicinal plant; micropropagation; somatic embryo conversion"@en . "43210" . "RIV/62156489:43210/05:00004744" . "0006-3134" . "CZ - \u010Cesk\u00E1 republika" . . "451;454" . . . . . . . "Havel, Ladislav" . "P(GA521/02/1129)" . . "We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 microM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 microM of 2,4-D and 4 microM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 microM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 microM)conntributed to their rapid conversion to plantlets."@cs . . "Biologia Plantarum" . "[0B5186E38F21]" . "Somatic embryogenesis from zygotic embryos of Schisandra chinensis" . "The article is in English. See original desription in the original language."@en . "3"^^ . "49" . . "3"^^ . "Somatic embryogenesis from zygotic embryos of Schisandra chinensis"@cs . . . "543480" . "3" . . "Vla\u0161\u00EDnov\u00E1, Helena" . "Sm\u00ED\u0161kov\u00E1, Alexandra" . . . "Somatic embryogenesis from zygotic embryos of Schisandra chinensis"@en . "Somatic embryogenesis from zygotic embryos of Schisandra chinensis" . . "We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 microM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 microM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 microM of 2,4-D and 4 microM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 microM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 microM)conntributed to their rapid conversion to plantlets." .