. "An increasing concentration of heavy metals in the environment is a serious problem for human and animal health protection and production of foodstuffs in many countries around the world. The aim of this paper was to suggest a new heavy metal biosensor based on interaction of metals (cadmium and zinc) with metallothionein, which belongs to group of intracellular, high molecular and cysteine-rich proteins binding heavy metals, using adsorptive transfer stripping (AdTS) differential pulse voltammetry (DPV). Primarily, we studied the electrochemical behaviour of MT on the surface of hanging mercury drop electrode by AdTS DPV. Perfect coverage of the electrode surface -- forming of the surface assembled monolayer -- was probably reached at time about 240 s for 10 mM protein concentration. The detection limits of the selected heavy metals (cadmium and zinc), which were analysed in the presence of the basic electrolyte -- 0.5 M NaCl (pH 6.4), were 250 fmole and 350 fmole in 5 ml drop, respectively. In addit" . "Study of metallothionein modified electrode surface behaviour in the presence of heavy metal ions - biosensor"@en . "43210" . "RIV/62156489:43210/05:00000273" . . . . "K\u00EDzek, Ren\u00E9" . . "Study of metallothionein modified electrode surface behaviour in the presence of heavy metal ions - biosensor"@en . . "RIV/62156489:43210/05:00000273!RIV06-GA0-43210___" . . . . "DPV; heavy metals; metallothionein"@en . "An increasing concentration of heavy metals in the environment is a serious problem for human and animal health protection and production of foodstuffs in many countries around the world. The aim of this paper was to suggest a new heavy metal biosensor based on interaction of metals (cadmium and zinc) with metallothionein, which belongs to group of intracellular, high molecular and cysteine-rich proteins binding heavy metals, using adsorptive transfer stripping (AdTS) differential pulse voltammetry (DPV). Primarily, we studied the electrochemical behaviour of MT on the surface of hanging mercury drop electrode by AdTS DPV. Perfect coverage of the electrode surface -- forming of the surface assembled monolayer -- was probably reached at time about 240 s for 10 mM protein concentration. The detection limits of the selected heavy metals (cadmium and zinc), which were analysed in the presence of the basic electrolyte -- 0.5 M NaCl (pH 6.4), were 250 fmole and 350 fmole in 5 ml drop, respectively. In addit"@cs . . "Studium metalothioneinem modifikovan\u00E9ho provrchu elektrody v p\u0159\u00EDtomnosti t\u011B\u017Ek\u00FDch kov\u016F-biosenzor"@cs . . "An increasing concentration of heavy metals in the environment is a serious problem for human and animal health protection and production of foodstuffs in many countries around the world. The aim of this paper was to suggest a new heavy metal biosensor based on interaction of metals (cadmium and zinc) with metallothionein, which belongs to group of intracellular, high molecular and cysteine-rich proteins binding heavy metals, using adsorptive transfer stripping (AdTS) differential pulse voltammetry (DPV). Primarily, we studied the electrochemical behaviour of MT on the surface of hanging mercury drop electrode by AdTS DPV. Perfect coverage of the electrode surface -- forming of the surface assembled monolayer -- was probably reached at time about 240 s for 10 mM protein concentration. The detection limits of the selected heavy metals (cadmium and zinc), which were analysed in the presence of the basic electrolyte -- 0.5 M NaCl (pH 6.4), were 250 fmole and 350 fmole in 5 ml drop, respectively. In addit"@en . "17" . "Trnkov\u00E1, Libu\u0161e" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Pot\u011B\u0161il, David" . "[43EB368C6A41]" . . "1649;1657" . "18" . "P(GP525/04/P132)" . "3"^^ . . "Zehn\u00E1lek, Josef" . "Petrlov\u00E1, Jitka" . . . "Study of metallothionein modified electrode surface behaviour in the presence of heavy metal ions - biosensor" . "Jelen, Franti\u0161ek" . . "Adam, Vojt\u011Bch" . . "Study of metallothionein modified electrode surface behaviour in the presence of heavy metal ions - biosensor" . "Studium metalothioneinem modifikovan\u00E9ho provrchu elektrody v p\u0159\u00EDtomnosti t\u011B\u017Ek\u00FDch kov\u016F-biosenzor"@cs . "545246" . "Sures, Bernd" . "1040-0397" . "9"^^ . "8"^^ . "Electroanalysis" .