"JP - Japonsko" . "Bioscience, Biotechnology and Biochemistry" . . . . "Yamada, M." . "Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302"@en . "1" . . "COPPER AMINE OXIDASE; TOPA QUINONE; HANSENULA-POLYMORPHA; CRYSTAL-STRUCTURE; CANDIDA-UTILIS; ENZYMES; BIOGENESIS; RESOLUTION; YEASTS"@en . . "RIV/61989592:15310/99:00007756!RIV09-MSM-15310___" . . . . . . . "15310" . "63" . . . . "6"^^ . "Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302"@en . "Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302" . "Purifikace a charakterizace methylaminoxidasy indukovan\u00E9 v Aspergillus niger AKU 3302"@cs . . "RIV/61989592:15310/99:00007756" . "751756" . . . "Toyama, H." . "Adachi, O." . "Crude extract of Aspergillus niger AKU 3302 mycelia incubated with methylamine showed a single amine oxidase activity band in a developed polyacrylamide gel that weakly cross-reacted with the antibody against a copper/topa quinone-containing amine oxidase (AO-II) from the same strain induced by n-butylamine. Since the organism cannot grow on methylamine and the already known quinoprotein amine oxidases of the organism cannot catalyze oxidation of methylamine, the organism was forced to produce another enzyme that could oxidize methylamine when the mycelia were incubated with methylamine. The enzyme was separated and purified from the already known two quinoprotein amine oxidases formed in the same mycelia. The purified enzyme showed a sharp symmetric sedimentation peak in analytical ultracentrifugation showing S-20,w(0) of 6.5s, The molecular mass of 133 kDa estimated by gel chromatography and 66.6 kDa found by SDS-PAGE confirmed the dimeric structure of the enzyme. The purified enzyme was pink in col" . . "P(ME 153), P(VS96021), Z(MSM 153100010), Z(MSM 153100013)" . "0916-8451" . "[AFBFB6642859]" . "Matsushita, K." . "Purification and characterization of methylamine oxidase induced in Aspergillus niger AKU 3302" . . "2"^^ . "Crude extract of Aspergillus niger AKU 3302 mycelia incubated with methylamine showed a single amine oxidase activity band in a developed polyacrylamide gel that weakly cross-reacted with the antibody against a copper/topa quinone-containing amine oxidase (AO-II) from the same strain induced by n-butylamine. Since the organism cannot grow on methylamine and the already known quinoprotein amine oxidases of the organism cannot catalyze oxidation of methylamine, the organism was forced to produce another enzyme that could oxidize methylamine when the mycelia were incubated with methylamine. The enzyme was separated and purified from the already known two quinoprotein amine oxidases formed in the same mycelia. The purified enzyme showed a sharp symmetric sedimentation peak in analytical ultracentrifugation showing S-20,w(0) of 6.5s, The molecular mass of 133 kDa estimated by gel chromatography and 66.6 kDa found by SDS-PAGE confirmed the dimeric structure of the enzyme. The purified enzyme was pink in col"@en . "Fr\u00E9bort, Ivo" . "Lemr, Karel" . "Mycelia pl\u00EDsn\u011B Aspergillus niger AKU 3302 inkubovan\u00E1 s methylaminem produkuj\u00ED enzym methylaminoxidasu, kter\u00FD p\u0159\u00EDslu\u0161\u00ED k chinoprotein\u016Fm. Enzym byl izolov\u00E1n chromatografick\u00FDmi metodami a byly ur\u010Deny jeho vlastnosti. Jedn\u00E1 se o dimer (2 x 67 kDa), kter\u00FD je zbarven r\u016F\u017Eov\u011B d\u00EDky p\u0159\u00EDtomnosti chinonov\u00E9ho kofaktoru. Methylaminoxidasa ochotn\u011B oxiduje methylamin, n-hexylamin a n-butylamin, nep\u016Fsob\u00ED na benzylamin, histamin a tyramin. Je inaktivov\u00E1na karbonylov\u00FDmi \u010Dinidly a \u010Dinidly chelatuj\u00EDc\u00EDmi dvojmocnou m\u011B\u010F. P\u0159\u00EDtomnost kofaktoru topachinonu byla prok\u00E1z\u00E1na spektroskopick\u00FDmi metodami. Pomoc\u00ED hmotnostn\u00ED spektrometrie byla z\u00EDsk\u00E1na data o \u010D\u00E1ste\u010Dn\u00E9 aminokyselinov\u00E9 sekvenci enzymu."@cs . "Purifikace a charakterizace methylaminoxidasy indukovan\u00E9 v Aspergillus niger AKU 3302"@cs . . "10"^^ . . . . . . .