"Xanthohumol attenuates tumour cell-mediated breaching of the lymphendothelial barrier and prevents intravasation and metastasis" . "0340-5761" . "Atanasov, Atanas" . . . "Raab, Ingrid" . . . "Xanthohumol attenuates tumour cell-mediated breaching of the lymphendothelial barrier and prevents intravasation and metastasis"@en . "7" . . "Krupitza, Georg" . . "Archives of Toxicology" . "Knasmueller, Siegfried" . "000320280000011" . "Krieger, Sigurd" . "Dirsch, Verena M" . "Health beneficial effects of xanthohumol have been reported, and basic research provided evidence for anti-cancer effects. Furthermore, xanthohumol was shown to inhibit the migration of endothelial cells. Therefore, this study investigated the anti-metastatic potential of xanthohumol. MCF-7 breast cancer spheroids which are placed on lymphendothelial cells (LECs) induce %22circular chemorepellent-induced defects%22 (CCIDs) in the LEC monolayer resembling gates for intravasating tumour bulks at an early step of lymph node colonisation. NF-kappa B reporter-, EROD-, SELE-, 12(S)-HETE- and adhesion assays were performed to investigate the anti-metastatic properties of xanthohumol. Western blot analyses were used to elucidate the mechanisms inhibiting CCID formation. Xanthohumol inhibited the activity of CYP, SELE and NF-kB and consequently, the formation of CCIDs at low micromolar concentrations. More specifically, xanthohumol affected ICAM-1 expression and adherence of MCF-7 cells to LECs, which is a prerequisite for CCID formation. Furthermore, markers of epithelial-to-mesenchymal transition (EMT) and of cell mobility such as paxillin, MCL2 and S100A4 were suppressed by xanthohumol. Xanthohumol attenuated tumour cell-mediated defects at the lymphendothelial barrier and inhibited EMT-like effects thereby providing a mechanistic explanation for the anti-intravasative/anti-metastatic properties of xanthohumol"@en . "Teichmann, Mathias" . "117556" . . "[6030C2F19D2D]" . "23"^^ . . "Viola, Katharina" . . . "Saiko, Philipp" . . "De Martin, Rainer" . "Szekeres, Thomas" . . "Giessrigl, Benedikt" . . "Kretschy, Nicole" . "Mikulits, Wolfgang" . "Mobility; Adhesion; Lymph endothelium; Cancer intravasation; Xanthohumol"@en . . "R\u00E1rov\u00E1, Lucie" . "Health beneficial effects of xanthohumol have been reported, and basic research provided evidence for anti-cancer effects. Furthermore, xanthohumol was shown to inhibit the migration of endothelial cells. Therefore, this study investigated the anti-metastatic potential of xanthohumol. MCF-7 breast cancer spheroids which are placed on lymphendothelial cells (LECs) induce %22circular chemorepellent-induced defects%22 (CCIDs) in the LEC monolayer resembling gates for intravasating tumour bulks at an early step of lymph node colonisation. NF-kappa B reporter-, EROD-, SELE-, 12(S)-HETE- and adhesion assays were performed to investigate the anti-metastatic properties of xanthohumol. Western blot analyses were used to elucidate the mechanisms inhibiting CCID formation. Xanthohumol inhibited the activity of CYP, SELE and NF-kB and consequently, the formation of CCIDs at low micromolar concentrations. More specifically, xanthohumol affected ICAM-1 expression and adherence of MCF-7 cells to LECs, which is a prerequisite for CCID formation. Furthermore, markers of epithelial-to-mesenchymal transition (EMT) and of cell mobility such as paxillin, MCL2 and S100A4 were suppressed by xanthohumol. Xanthohumol attenuated tumour cell-mediated defects at the lymphendothelial barrier and inhibited EMT-like effects thereby providing a mechanistic explanation for the anti-intravasative/anti-metastatic properties of xanthohumol" . "15310" . "Xanthohumol attenuates tumour cell-mediated breaching of the lymphendothelial barrier and prevents intravasation and metastasis" . . "J\u00E4ger, Walter" . "Grusch, Michael" . . "Vonach, Caroline" . "Dolznig, Helmut" . "87" . . . "12"^^ . "Xanthohumol attenuates tumour cell-mediated breaching of the lymphendothelial barrier and prevents intravasation and metastasis"@en . "Huttary, Nicole" . "Jarukamjorn, Kanokwan" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "3"^^ . . "Kopf, Sabine" . "RIV/61989592:15310/13:33148296" . "RIV/61989592:15310/13:33148296!RIV14-MSM-15310___" . "10.1007/s00204-013-1028-2" . "http://link.springer.com/article/10.1007%2Fs00204-013-1028-2" . "P(ED0007/01/01)" . "Strnad, Miroslav" . "Mikulits, Wolfgang" .