"V\u00E1vrov\u00E1, Aneta" . "58870" . . "[D7AB5E358DB5]" . "3"^^ . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . . "Dvo\u0159\u00E1k, Zden\u011Bk" . . . . "000322112200003" . "3"^^ . "Vrzal, Radim" . "http://onlinelibrary.wiley.com/doi/10.1002/elps.201300079/full" . . . "RIV/61989592:15310/13:33147707" . . . "0173-0835" . "A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element"@en . "Electrophoresis" . "15310" . . "A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element" . "The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ??P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences."@en . "34" . . . "6"^^ . "A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element" . . "The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA-protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ??P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences." . "RIV/61989592:15310/13:33147707!RIV14-GA0-15310___" . "13" . "A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to CYP3A4 response element"@en . . . . "P(GA13-01809S), P(GAP303/12/0472), S" . "Response elements; Pregnane X receptor; Electrophoretic mobility shift assay; Drug metabolism; Cytochrome P450"@en . . . . "10.1002/elps.201300079" . .