. . "8" . . "\u0158ehulka, Pavel" . . "Sedl\u00E1\u0159ov\u00E1, Michaela" . "Marchetti-Deschmann, Martina" . "Chalupov\u00E1, Jana" . "Helmel, Michaela" . "I, P(ED0007/01/01), P(MEB061003), Z(MSM6198959215)" . . "Journal of Mass Spectrometry" . "This study deals with a methodology for MALDI-TOF MS-based identification of downy and powdery mildews representing obligate biotrophic parasites of crop plants. Experimental approaches for the MS analyses were optimized using Bremia lactucae, cause of lettuce downy mildew, and Oidium neolycopersici, cause of tomato powdery mildew. This involved optimization of sample preparation protocols and tuning of conditions during the measurements. The best peptide/protein profiles (in terms of signal-to-noise ratio and number of peaks) were obtained by combining ferulic and sinapinic acids as a mixed MALDI matrix. A pretreatment of the spore cell wall with hydrolases was successfully introduced prior to MS measurements to obtain more pronounced signals. Finally, a novel procedure was developed for direct mass spectra acquisition from infected plant leaves." . . "1076-5174" . . "Allmaier, Guenter" . . . "This study deals with a methodology for MALDI-TOF MS-based identification of downy and powdery mildews representing obligate biotrophic parasites of crop plants. Experimental approaches for the MS analyses were optimized using Bremia lactucae, cause of lettuce downy mildew, and Oidium neolycopersici, cause of tomato powdery mildew. This involved optimization of sample preparation protocols and tuning of conditions during the measurements. The best peptide/protein profiles (in terms of signal-to-noise ratio and number of peaks) were obtained by combining ferulic and sinapinic acids as a mixed MALDI matrix. A pretreatment of the spore cell wall with hydrolases was successfully introduced prior to MS measurements to obtain more pronounced signals. Finally, a novel procedure was developed for direct mass spectra acquisition from infected plant leaves."@en . "9"^^ . . "MALDI-based intact spore mass spectrometry of downy and powdery mildews" . "7"^^ . . . . "47" . . . . . "MALDI-based intact spore mass spectrometry of downy and powdery mildews"@en . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "protein marker; plant pathogen; MALDI-TOF; intact cell/spore mass spectrometry; biotyping"@en . "http://onlinelibrary.wiley.com/doi/10.1002/jms.3046/pdf" . . "3"^^ . "147898" . "MALDI-based intact spore mass spectrometry of downy and powdery mildews" . . "10.1002/jms.3046" . "RIV/61989592:15310/12:33142960" . . "\u0160ebela, Marek" . . . "MALDI-based intact spore mass spectrometry of downy and powdery mildews"@en . "[74B1978FC03F]" . "15310" . "RIV/61989592:15310/12:33142960!RIV13-MSM-15310___" . . "000307932000005" .