"Secondary processes in atmospheric pressure chemical ionization-ion trap mass spectrometry: a case study of orotic acid"@en . "Bedn\u00E1\u0159, Petr" . . "hydrogen-deuterium exchange; ion-molecule reactions; collision-induced dissociation; atmospheric pressure chemical ionization; mass spectrometry; orotic acid"@en . . . . . "6" . . "Atmospheric pressure chemical ionization is known for producing unusual artifacts of the ionization process in some cases. In this work, processes occuring in atmospheric pressure chemical ionization/MS of orotic acid that afforded ions accompanying protonated and deprotonated orotic acid molecules in the spectra were studied. Two processes ran in parallel in the ion source: decarboxylation of neutral orotic acid and collision-induced dissociation of its protonated or deprotonated form. A procedure discerning pre-ionization decomposition and post-ionization dissociation by manipulating ion source parameters was proposed. Experiments with isotopically labeled solvents confirmed ion-molecule reactions of the product of collision-induced dissociation of protonated orotic acid with solvent molecules in the ion source and even under vacuum in the ion trap."@en . "Secondary processes in atmospheric pressure chemical ionization-ion trap mass spectrometry: a case study of orotic acid" . . "15310" . "000305528300007" . . . "Fry\u010D\u00E1k, Petr" . "6"^^ . "RIV/61989592:15310/12:33142287" . "I, P(ED2.1.00/03.0058), P(GAP206/12/1150), P(ME10013)" . . "Lemr, Karel" . "1076-5174" . . . . . . "167157" . . "Jirkovsk\u00FD, Jarom\u00EDr" . . . "[F0828B3CEAFB]" . . . "Journal of Mass Spectrometry" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Havl\u00ED\u010Dek, Vladim\u00EDr" . . "Ranc, V\u00E1clav" . "Secondary processes in atmospheric pressure chemical ionization-ion trap mass spectrometry: a case study of orotic acid"@en . "Atmospheric pressure chemical ionization is known for producing unusual artifacts of the ionization process in some cases. In this work, processes occuring in atmospheric pressure chemical ionization/MS of orotic acid that afforded ions accompanying protonated and deprotonated orotic acid molecules in the spectra were studied. Two processes ran in parallel in the ion source: decarboxylation of neutral orotic acid and collision-induced dissociation of its protonated or deprotonated form. A procedure discerning pre-ionization decomposition and post-ionization dissociation by manipulating ion source parameters was proposed. Experiments with isotopically labeled solvents confirmed ion-molecule reactions of the product of collision-induced dissociation of protonated orotic acid with solvent molecules in the ion source and even under vacuum in the ion trap." . . "RIV/61989592:15310/12:33142287!RIV13-MSM-15310___" . "47" . "7"^^ . "10.1002/jms.3006" . "Secondary processes in atmospheric pressure chemical ionization-ion trap mass spectrometry: a case study of orotic acid" . . "4"^^ . . .