. "15310" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . . "RIV/61989592:15310/10:10212711" . . . "Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress" . "Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple types of cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts)."@en . "Luhov\u00E1, Lenka" . . . . . . . "7"^^ . . "Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress"@en . "61" . . "[8DBDAE9EE3C1]" . "Journal of Experimenal Botany" . . . . "Protiv\u00E1nkov\u00E1, Iva" . . "Piterkov\u00E1, Jana" . . "000279003600015" . "RIV/61989592:15310/10:10212711!RIV11-MSM-15310___" . . . "Lebeda, Ale\u0161" . . . "Ond\u0159ej, Vladan" . "9" . "7"^^ . . "Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress" . "Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress"@en . "Navr\u00E1tilov\u00E1, Bo\u017Eena" . "0022-0957" . "P(2E08018), Z(MSM6198959215)" . "Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple types of cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts)." . "284206" . "7"^^ . "Sedl\u00E1\u0159ov\u00E1, Michaela" . "protoplasts; oxidative stress; nuclear organization; chromatin"@en .