"Journal of Cellular Biochemistry" . "106" . . "Maurer, Margarita" . "Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment"@en . . . "RIV/61989592:15310/09:00010544!RIV10-MSM-15310___" . "0730-2312" . . "7"^^ . . . "Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment" . . "Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment" . . . . "Exposure of asynchronously growing human HeLa cervical carcinoma cells to roscovitine (ROSC), a selective cyclin-dependent kinases (CDKs) inhibitor, arrests their progression at the transition between G(2)/M and/or induces apoptosis. The outcome depends on the ROSC concentration. At higher close ROSC represses HPV-encoded E7 oncoprotein and initiates caspase-dependent apoptosis. Inhibition of the site-specific phosphorylation of survivin and Bad, occurring at high-dose ROSC treatment, precedes the onset of apoptosis and seems to be a prerequisite for cell death. Considering the fact that in HeLa cells the G(1)/S restriction checkpoint is abolished by E7, we addressed the question whether the inhibition of CDKs by pharmacological inhibitors in synchronized cells would be able to block the cell-cycle in G, phase. For this purpose, we attempted to synchronize cells by serum withdrawal or by blocking of the mitotic apparatus using nocodazole. Unlike human MCF-7 cells, HeLa cells do not undergo G, block af"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Walzi, Eva" . "15310" . "HUMAN-PAPILLOMAVIRUS TYPE-16; KINASE INHIBITORS OLOMOUCINE; CYC202 R-ROSCOVITINE; CDK INHIBITORS; RETINOBLASTOMA PROTEIN; CENTROSOME DUPLICATION; THERAPEUTIC TARGET; INDUCED APOPTOSIS; CRYSTAL-STRUCTURE; UP-REGULATION"@en . "[21C07857AEF0]" . . "1"^^ . "5" . "332356" . "19"^^ . . . . "Kry\u0161tof, Vladim\u00EDr" . "Wesierska-Gadek, Jozefa" . . "Wandl, Stefanie" . . . "Borza, Andreea" . "Komina, Oxana" . "Z(AV0Z50380511), Z(MSM6198959216)" . "Exposure of asynchronously growing human HeLa cervical carcinoma cells to roscovitine (ROSC), a selective cyclin-dependent kinases (CDKs) inhibitor, arrests their progression at the transition between G(2)/M and/or induces apoptosis. The outcome depends on the ROSC concentration. At higher close ROSC represses HPV-encoded E7 oncoprotein and initiates caspase-dependent apoptosis. Inhibition of the site-specific phosphorylation of survivin and Bad, occurring at high-dose ROSC treatment, precedes the onset of apoptosis and seems to be a prerequisite for cell death. Considering the fact that in HeLa cells the G(1)/S restriction checkpoint is abolished by E7, we addressed the question whether the inhibition of CDKs by pharmacological inhibitors in synchronized cells would be able to block the cell-cycle in G, phase. For this purpose, we attempted to synchronize cells by serum withdrawal or by blocking of the mitotic apparatus using nocodazole. Unlike human MCF-7 cells, HeLa cells do not undergo G, block af" . "RIV/61989592:15310/09:00010544" . . . . . . "Outcome of Treatment of Human HeLa Cervical Cancer Cells With Roscovitine Strongly Depends on the Dosage and Cell Cycle Status Prior to the Treatment"@en . .