. . "Lemr, Karel" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "Knob, Radim" . "6"^^ . . "Capillary electrophoresis; Desorption electrospray; Identification; Mass spectrometry; Microorganism"@en . . "Assessment of CE for the identification of microorganisms"@en . "Petr, Jan" . "3" . "Hinnerov\u00E1, Petra" . . . "Assessment of CE for the identification of microorganisms"@en . "RIV/61989592:15310/09:00010411" . "RIV/61989592:15310/09:00010411!RIV10-MSM-15310___" . . "Fr\u00E9bort, Ivo" . "11"^^ . . "11"^^ . "The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris-citrate-fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post-separation cultivation and sequential analysis was developed. Off-line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells." . "Assessment of CE for the identification of microorganisms" . . . "Kowalska, Marta" . "Ryparov\u00E1, Olga" . . "0173-0835" . . "Assessment of CE for the identification of microorganisms" . . "S, Z(MSM6198959216)" . "Znaleziona, Joanna" . . "The assessment of capillary electrophoresis for separation and identification of microorganisms is described in this article. The work brings a comparison of the application of uncoated capillaries modified with poly(ethylene oxide) and coated capillaries for the separation of model microorganisms Saccharomyces cerevisiae and Escherichia coli with Tris-borate-EDTA and Tris-citrate-fructose as electrolytes. The best separation was achieved in the coated capillary using 1 mM Tris-citrate-fructose buffer, pH 6.9. A simple identification based on the migration time and UV spectrum was found unsatisfactory and thus a method based on a post-separation cultivation and sequential analysis was developed. Off-line combination with mass spectrometric analysis with the use of desorption electrospray was shown to be an interesting alternative in a study of microorganisms. Mass spectra allowed distinguishing among analyzed cells."@en . . "[62B87B910098]" . "Maier, V\u00EDt\u011Bzslav" . "Znaleziona, Joanna" . "304181" . . "Ranc, V\u00E1clav" . . "Electrophoresis" . "15310" . "Kowalska, Marta" . . . . . . . . "\u0160ev\u010D\u00EDk, Juraj" . "30" . .