. "27" . . . "Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L." . "5"^^ . . . . "cell cycle; chromatin; plant protoplasts; subtelomeric repeats"@en . "Dole\u017Ealov\u00E1, Ivana" . "This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a" . "Lebeda, Ale\u0161" . "[7116102A5279]" . . . "6"^^ . "Molecules and Cells" . "RIV/61989592:15310/09:00010056!RIV10-MSM-15310___" . . . . "N\u00E1dvorn\u00EDk, Petr" . "6"^^ . . . "Z(MSM6198959215)" . "15310" . . "Ond\u0159ej, Vladan" . . "Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L."@en . "KR - Korejsk\u00E1 republika" . "Kitner, Miloslav" . "RIV/61989592:15310/09:00010056" . . "307038" . "This paper reports on the structural rearrangement of satellite DNA type I repeats and heterochromatin during the dedifferentiation and cell cycling of mesophyll protoplasts of cucumber (Cucumis sativus). These repeats were localized in the telomeric heterochromatin of cucumber chromosomes and in the chromocenters of interphase nuclei. The dramatic reduction of heterochromatin involves decondensation of subtelomeric repeats in freshly isolated protoplasts; however, there are not a great many remarkable changes in the expression profile. In spite of that, reformation of the chromocenters, occurring 48 h after protoplast isolation, is accompanied by recondensation of satellite DNA type I; however, only partial reassembly of these repeats was revealed. In this study, FISH and a flow cytometry assay show a correlation between the partial chromocenter and the repeats reassembly, and with the reentry of cultivated protoplasts into the cell cycle and first cell division. After that, divided cells displayed a"@en . . "1016-8478" . . . "Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L." . "4" . . "Navr\u00E1tilov\u00E1, Bo\u017Eena" . . "Chromatin Structural Rearrangement during Dedifferentiation of Protoplasts of Cucumis sativus L."@en .