. "Ranc, V\u00E1clav" . "1469-0667" . "4"^^ . "Lemr, Karel" . "Nano-desorption electrospray and kinetic method in chiral analysis of drugs in whole human blood samples"@en . . . . . . "4"^^ . "14" . . "Havl\u00ED\u010Dek, Vladim\u00EDr" . "381594" . "nano-desorption electrospray; mass spectrometry; kinetic method; chiral analysis; drug analysis; human blood samples"@en . "RIV/61989592:15310/08:00005427!RIV10-MSM-15310___" . . "Nano-desorption electrospray and kinetic method in chiral analysis of drugs in whole human blood samples"@en . "European Journal of Mass Spectrometry" . . . . . . "Nano-desorption electrospray and kinetic method in chiral analysis of drugs in whole human blood samples" . . . "A home-made nano-desorption electrospray ionization (nano-DESI) device and the kinetic method were tested in chiral analysis of model clinical samples containing enantiomers of one of three pharmaceutically important compounds: dihydroxyphenylalanine (DOPA), ephedrine and ibuprofen. The initial evaluation of chiral systems was carried out by direct infusion of solution mixtures (analyte/central metal/chiral reference ligand) to a standard electrospray ionization (ESI) source. Cu(II) was used as a central metal for all. analytes, L-phenylalanine was applied as a chiral reference ligand for DOPA, whereas L-tryptophan was used for the other two analytes. Then, the ESI source was substituted by a nano-DESI source and dried spots of 1 mu L samples of whole human blood spiked with individual drugs were successfully analyzed without any pre-treatment. Irrespective of a laborious initial nano-DESI set-up, the combination of the kinetic method with nano-desorption electrospray has, for the first time, been dem"@en . . "[2087044EC651]" . . "Bedn\u00E1\u0159, Petr" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "RIV/61989592:15310/08:00005427" . . "7"^^ . . "Nano-desorption electrospray and kinetic method in chiral analysis of drugs in whole human blood samples" . . "P(GA203/07/0765), S, Z(MSM6198959216)" . . . "A home-made nano-desorption electrospray ionization (nano-DESI) device and the kinetic method were tested in chiral analysis of model clinical samples containing enantiomers of one of three pharmaceutically important compounds: dihydroxyphenylalanine (DOPA), ephedrine and ibuprofen. The initial evaluation of chiral systems was carried out by direct infusion of solution mixtures (analyte/central metal/chiral reference ligand) to a standard electrospray ionization (ESI) source. Cu(II) was used as a central metal for all. analytes, L-phenylalanine was applied as a chiral reference ligand for DOPA, whereas L-tryptophan was used for the other two analytes. Then, the ESI source was substituted by a nano-DESI source and dried spots of 1 mu L samples of whole human blood spiked with individual drugs were successfully analyzed without any pre-treatment. Irrespective of a laborious initial nano-DESI set-up, the combination of the kinetic method with nano-desorption electrospray has, for the first time, been dem" . . . "6" . "15310" .