"15310" . "Isoenzymy konven\u010Dn\u00ED protein dinasy C podl\u00E9haj\u00ED defosforylaci v neutrofil\u016Fm podobn\u00FDch HL-60 bu\u0148k\u00E1ch inkubovan\u00FDch s chelerythrinem a sanguinarinem."@cs . . "4"^^ . . "0742-2091" . "Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine"@en . "The quaternary benzo[c]phenanthridine alkaloid chelerythrine is widely used as an inhibitor of protein kinase C (PKC). However, in biological systems chelerythrine interacts with an array of proteins. In this study, we examined the effects of chelerythrine and sanguinarine on conventional PKCs (cPKCs) and PKC upstream kinase, phosphoinositide-dependent protein kinase 1 (PDK1), under complete inhibition conditions of PKC-dependent oxidative burst. In neutrophil-like HL-60 cells, sanguinarine and chelerythrine inhibited N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, and A23187-induced oxidative burst with IC50 values not exceeding 4.6 mu mol/L, but the inhibition of PMA-stimulated cPKC activity in intact cells required at least fivefold higher alkaloid concentrations. At concentrations below 10 mu mol/L, sanguinarine and chelerythrine prevented phosphorylation of similar to 80 kDa protein and sequestered similar to 60 kDa phosphoprotein in cytosol. Moreover, neither sanguinarine nor chele"@en . "3"^^ . "P(GP303/06/P193), Z(MSM6198959216)" . . "Cell Biology and Toxicology" . "The quaternary benzo[c]phenanthridine alkaloid chelerythrine is widely used as an inhibitor of protein kinase C (PKC). However, in biological systems chelerythrine interacts with an array of proteins. In this study, we examined the effects of chelerythrine and sanguinarine on conventional PKCs (cPKCs) and PKC upstream kinase, phosphoinositide-dependent protein kinase 1 (PDK1), under complete inhibition conditions of PKC-dependent oxidative burst. In neutrophil-like HL-60 cells, sanguinarine and chelerythrine inhibited N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, and A23187-induced oxidative burst with IC50 values not exceeding 4.6 mu mol/L, but the inhibition of PMA-stimulated cPKC activity in intact cells required at least fivefold higher alkaloid concentrations. At concentrations below 10 mu mol/L, sanguinarine and chelerythrine prevented phosphorylation of similar to 80 kDa protein and sequestered similar to 60 kDa phosphoprotein in cytosol. Moreover, neither sanguinarine nor chele" . "361289" . "Ulrichov\u00E1, Jitka" . "RIV/61989592:15310/08:00005343" . "Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine" . . . "Isoenzymy konven\u010Dn\u00ED protein dinasy C podl\u00E9haj\u00ED defosforylaci v neutrofil\u016Fm podobn\u00FDch HL-60 bu\u0148k\u00E1ch inkubovan\u00FDch s chelerythrinem a sanguinarinem."@cs . "[EC956DDA4268]" . "Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine"@en . . "24" . . . "Dvo\u0159\u00E1k, Zden\u011Bk" . . . "Kvart\u00E9rn\u00ED benzo[c]fenanthridinov\u00FD alkaloid chelerythrin je \u0161iroce vyu\u017E\u00EDv\u00E1n jako inhibitor protein kinasy C (PKC). V biologick\u00FDch syst\u00E9mech ale chelerythrin interaguje s plej\u00E1dou protein\u016F. V t\u00E9to studii jsme zkoumali \u00FA\u010Dinky chelerythrinu a sanguinarinu na konven\u010Dn\u00ED PKCs (cPKCs) a PKC upstream kinasu, fosfoinositidedependent protein kinasu 1 (PDK1), za podm\u00EDnek kompletn\u00ED inhibice PKC-dependentn\u00EDho oxidativn\u00EDho vzplanut\u00ED. V neutrofil\u016Fm podobn\u00FDch bu\u0148k\u00E1ch HL-60 cells, inhibovaly sanguinarin a chelerythrin N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, a A23187-indukovan\u00E9 oxidativn\u00ED vzplanut\u00ED, s hodnotami IC50 nep\u0159esahuj\u00EDc\u00ED 4.6 \u03BCmol/L, ale inhibice PMA-stimulovan\u00E9 cPKC aktivity v intaktn\u00EDch bu\u0148k\u00E1ch vy\u017Eadovala nejm\u00E9n\u011B p\u011Btkr\u00E1t vy\u0161\u0161\u00ED koncentraci alkaloidu. Sanguinarin a chelerythrin br\u00E1nily fosforylaci 80 kDa proteinu a zadr\u017Eovaly 60 kDa fosfoproteid v cytosolu v koncentrac\u00EDch pod 10 \u03BCmol/L. Nav\u00EDc ani sanguinarin ani chelerythrin neblokovaly PMA-stimulovanou translokaci a"@cs . "Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine" . . "NL - Nizozemsko" . "15"^^ . "Modriansk\u00FD, Martin" . . . . . . . "RIV/61989592:15310/08:00005343!RIV09-MSM-15310___" . . "Vrba, Ji\u0159\u00ED" . . . "1" . "chelerythrine; HL-60 cells; oxidative burst; phosphoinositide-dependent protein kinase 1; protein kinase C; sanguinarine"@en . . .