"We have developed enzyme-linked immunosorbent assays (ELISAs) for measuring 24-epicastasterone and related brassinolide analogs, with detection ranges of 0.005 to 50 pmoles. Polyclonal antibodies used in these assays were raised against 24-epicastasterone carboxymethyloxime-bovine serum albumin conjugates and were found to have high specificity for 24-epibrassinosteroids. Natural brassinosteroids (BRs), such as brassinolide and 24-epibrassinolide, exhibited relatively high cross-reactivities with the generated antibodies, whereas other BR analogs with beta-oriented hydroxyl groups at C-2, C-3, C-22, and C23 lacked immunoreactivity. Through the use of internal standardization, dilution assays, recovery of authentic [H-3]24-epicastasterone, and immunohistograms, the ELISAs have been shown to be applicable for estimating 24-epibrassinosteroid levels in crude plant extracts. To analyze brassinosteroids in tissues from young bean (Phaseolus vulgaris L., cv. Pinto), Daucus carota ssp.sativus plants and Arab"@en . "New techniques for the estimation of naturally occurring brassinosteroids"@en . "[1DC6997EE216]" . . . "7"^^ . . . "RIV/61989592:15310/07:00010651!RIV10-MSM-15310___" . "Okle\u0161\u0165kov\u00E1, Jana" . . "New techniques for the estimation of naturally occurring brassinosteroids"@en . . "1" . "New techniques for the estimation of naturally occurring brassinosteroids" . . "Journal of Plant Growth Regulation" . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . "antibodies; Arabidopsis; brassinosteroids; enzyme immunoassay; HPLC-MS; rape pollen"@en . "4"^^ . "14"^^ . "Strnad, Miroslav" . "0721-7595" . . "Fuksov\u00E1, Kv\u011Bta" . . "New techniques for the estimation of naturally occurring brassinosteroids" . . "H\u00E9nykov\u00E1, Eva" . . "15310" . "Z(AV0Z40550506), Z(AV0Z50380511), Z(MSM6198959216)" . . "Nov\u00E1k, Ond\u0159ej" . . "437235" . "\u0160\u00ED\u0161a, Miroslav" . . . . . "We have developed enzyme-linked immunosorbent assays (ELISAs) for measuring 24-epicastasterone and related brassinolide analogs, with detection ranges of 0.005 to 50 pmoles. Polyclonal antibodies used in these assays were raised against 24-epicastasterone carboxymethyloxime-bovine serum albumin conjugates and were found to have high specificity for 24-epibrassinosteroids. Natural brassinosteroids (BRs), such as brassinolide and 24-epibrassinolide, exhibited relatively high cross-reactivities with the generated antibodies, whereas other BR analogs with beta-oriented hydroxyl groups at C-2, C-3, C-22, and C23 lacked immunoreactivity. Through the use of internal standardization, dilution assays, recovery of authentic [H-3]24-epicastasterone, and immunohistograms, the ELISAs have been shown to be applicable for estimating 24-epibrassinosteroid levels in crude plant extracts. To analyze brassinosteroids in tissues from young bean (Phaseolus vulgaris L., cv. Pinto), Daucus carota ssp.sativus plants and Arab" . . "26" . "Kohout, Ladislav" . . "RIV/61989592:15310/07:00010651" . . .