"On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry"@en . "[AAF7A3136358]" . . . . . "0003-2700" . . . "15310" . . . . "439515" . "On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry" . . "RIV/61989592:15310/07:00004838!RIV10-MSM-15310___" . "Schug, Kevin A." . "On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry" . . "Fry\u010D\u00E1k, Petr" . "14" . "Z(MSM6198959216)" . "A new method for determination of dissociation constants (K-d) using on-line titration by electrospray ionization mass spectrometry is presented. Unlike in common titration experiments, where a set of discrete solutions with a fixed concentration of host and increasing concentration of guest is measured, here a continuous Gaussian concentration profile of guest, formed by band-broadening dispersion during passage through a long tubing, is utilized. An equation allowing access to dissociation constant values from experimental data fit to a 1:1 binding model was derived and incorporated into an in-house-written computer program for automated data processing. The new method is demonstrated for noncovalent complexes of cinchona alkaloid carbamate chiral selectors with N-dinitrobenzoylleucine enantiomers and a series of cyclodextrins with sulfonated azo dyes." . "On-line dynamic titration: Determination of dissociation constants for noncovalent complexes using gaussian concentration profiles by electrospray ionization mass spectrometry"@en . "A new method for determination of dissociation constants (K-d) using on-line titration by electrospray ionization mass spectrometry is presented. Unlike in common titration experiments, where a set of discrete solutions with a fixed concentration of host and increasing concentration of guest is measured, here a continuous Gaussian concentration profile of guest, formed by band-broadening dispersion during passage through a long tubing, is utilized. An equation allowing access to dissociation constant values from experimental data fit to a 1:1 binding model was derived and incorporated into an in-house-written computer program for automated data processing. The new method is demonstrated for noncovalent complexes of cinchona alkaloid carbamate chiral selectors with N-dinitrobenzoylleucine enantiomers and a series of cyclodextrins with sulfonated azo dyes."@en . . . . . . "79" . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Analytical Chemistry" . . "2"^^ . "RIV/61989592:15310/07:00004838" . "7"^^ . "PROTEIN-LIGAND INTERACTIONS; AMYLOID-BETA-PEPTIDE; MOLECULAR RECOGNITION; BINDING INTERACTIONS; CHIRAL RECOGNITION; ANION-EXCHANGERS; N-METHYLPYRROLE; AMINO-ACIDS; RNA; ENANTIOSELECTIVITY"@en . . "1"^^ . . .