. . "Hlobilkov\u00E1, Alice" . . "\u0160v\u00E1chov\u00E1, Michaela" . "Kol\u00E1\u0159, Zden\u011Bk" . . "15310" . . . "Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells" . . . "504532" . . . "Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN" . "GR - \u0158eck\u00E1 republika" . . "8"^^ . . "0250-7005" . . . . . "Knillov\u00E1, Jana" . "Anticancer Research" . . . . "P(GA204/01/0488), P(NC6779), P(NR7828), Z(AV0Z50380511), Z(MSM6198959216)" . . . "26" . "RIV/61989592:15310/06:00010655!RIV10-MSM-15310___" . . . . "5"^^ . "Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells"@en . "Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells" . "[DA1BB8952A68]" . "Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN"@en . "Skypalov\u00E1, Petra" . "Kry\u0161tof, Vladim\u00EDr" . . . "6"^^ . . . . "Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells"@en . . "breast cancer cell lines; cell cycle; phosphatase activity; PKB/Akt; PTEN; tumour suppressor; p27(Kip1); p21(Waf1/Cip1); p53 and pRb"@en . "2A" . "RIV/61989592:15310/06:00010655" .