"0731-7085" . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "A rapid clean-up procedure based on solid-phase extraction (SPE) and HPLC determination of lincomycin in premixes with UV detection is described. After extraction of lincomycin from premix with extraction solvent the extract is applied to OASIS HLB column treated with methanol and water. Lincomycin is eluted with methanol and effluent is analysed on analytical column (phenyl) using mobile phase consists 0.2% phosphoric acid in water and acetonitrile (875:125, v/v). Detection is performed at 208 nm. Quantitation is carried out using external standard. The mean recovery of lincomycin was 105.0 +/- 7.3%, in concentration range of 250-750 mg k,(-1), and 99.8 +/- 3.7%, in concentration range of 10,000-150,000mg kg(-1). The limit of determination, based on a signal-to-noise ratio of 10:1, was 5.2mg kg(-1). LC-MS/MS confirmation of lincomycin is also presented. Identification was performed by monitoring two pairs of multiple reaction monitoring ions from the parent ions (m/z 407.2 -> 126.1 and 407.2 -"@en . "[6E26075097FB]" . . "40" . "Journal of Pharmaceutical and Biomedical Analysis" . "RIV/61989592:15310/06:00003365!RIV07-MSM-15310___" . "Z(MSM6198959216)" . "A rapid clean-up procedure based on solid-phase extraction (SPE) and HPLC determination of lincomycin in premixes with UV detection is described. After extraction of lincomycin from premix with extraction solvent the extract is applied to OASIS HLB column treated with methanol and water. Lincomycin is eluted with methanol and effluent is analysed on analytical column (phenyl) using mobile phase consists 0.2% phosphoric acid in water and acetonitrile (875:125, v/v). Detection is performed at 208 nm. Quantitation is carried out using external standard. The mean recovery of lincomycin was 105.0 +/- 7.3%, in concentration range of 250-750 mg k,(-1), and 99.8 +/- 3.7%, in concentration range of 10,000-150,000mg kg(-1). The limit of determination, based on a signal-to-noise ratio of 10:1, was 5.2mg kg(-1). LC-MS/MS confirmation of lincomycin is also presented. Identification was performed by monitoring two pairs of multiple reaction monitoring ions from the parent ions (m/z 407.2 -> 126.1 and 407.2 -" . "981-986" . . "HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation" . . . . . "Dou\u0161a, Michal" . "478308" . "1"^^ . "HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation" . . . "RIV/61989592:15310/06:00003365" . . "Halama, Michal" . . "Lemr, Karel" . "4"^^ . . "HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation"@en . "HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation"@en . "HPLC stanoven\u00ED linkomycinu v premixech a krmn\u00FDch sm\u011Bs\u00EDch s pou\u017Eit\u00EDm solid-phase extrakce na HLB OASIS a LC-MS/MS potvrzen\u00ED"@cs . "Sika\u010D, Zden\u011Bk" . . "HPLC stanoven\u00ED linkomycinu v premixech a krmn\u00FDch sm\u011Bs\u00EDch s pou\u017Eit\u00EDm solid-phase extrakce na HLB OASIS a LC-MS/MS potvrzen\u00ED"@cs . "15310" . "HPLC determination; LC-MS/MS confirmation; lincomycin; premix; validation"@en . "6"^^ . . . "4" . "Rychl\u00E1 procedura zalo\u017Een\u00E1 na solid-phase extrakci a HPLC stanoven\u00ED linkomycinu v premixech s UV detekc\u00ED je v pr\u00E1ci pops\u00E1na. Po extrakci linkomycinu pomoc\u00ED extrak\u010Dn\u00EDho \u010Dinidla je extrakt nast\u0159\u00EDknut na OASIS HLB kolonu. Linkomycin je eluov\u00E1n metanolem a eluent je analyzov\u00E1n na analytick\u00E9 kolon\u011B s pou\u017Eit\u00EDm mobiln\u00ED f\u00E1ze slo\u017Een\u00E9 z 0,2 % kyseliny fosfore\u010Dn\u00E9 ve sm\u011Bsi vody a acetonitrilu. Detekce je prov\u00E1d\u011Bna p\u0159i 208 nm.Ke kvantifikaci se pou\u017E\u00EDv\u00E1 metoda vn\u011Bj\u0161\u00EDho standardu. Pr\u016Fm\u011Brn\u00E1 v\u00FDt\u011B\u017Enost byla 105,0 +/- 7,3 % v koncentra\u010Dn\u00EDm rozmez\u00ED 250-750 mg/kg a 99,8 +/- 3,7 % v koncentra\u010Dn\u00EDm rozmez\u00ED 10000-150000 mg/kg. Mez detekce ur\u010Den\u00E1 pom\u011Brem sign\u00E1l ku \u0161umu 10:1 byla 5,2 mg/kg. LC-MS/MS konfirmace linkomycinu je tak\u00E9 prezentov\u00E1na."@cs .