"RIV/61989592:15310/06:00003355" . "Rozli\u0161en\u00ED izomer\u016F hmotnostn\u00ED spektrometri\u00ED s vyu\u017Eit\u00EDm kinetick\u00E9 metody"@cs . "498091" . . . . "5"^^ . "0009-2770" . "5"^^ . . "RIV/61989592:15310/06:00003355!RIV10-MSM-15310___" . . "Anal\u00FDza chir\u00E1ln\u00EDch l\u00E1tek pat\u0159\u00ED vzhledem ke sv\u00E9mu v\u00FDznamu pro oborz bezprost\u0159edn\u011B ovliv\u0148uj\u00EDc\u00ED kvalitu lidsk\u00E9ho \u017Eivota (nap\u0159. v\u00FDvoj a v\u00FDroba l\u00E9\u010Div) k d\u016Fle\u017Eit\u00FDm a st\u00E1le se rozv\u00EDjej\u00EDc\u00EDm odv\u011Btv\u00EDm analytick\u00E9 chemie. Mezi rutinn\u011B pou\u017E\u00EDvan\u00E9 metody v t\u00E9to oblasti pat\u0159\u00ED zejm\u00E9na vysoko\u00FA\u010Dinn\u00E1 kapalinov\u00E1 chromatografie a kapil\u00E1rn\u00ED elektrofor\u00E9za. Hmotnostn\u00ED spektrometrie se st\u00E1v\u00E1 b\u011B\u017Enou sou\u010D\u00E1st\u00ED mnoha laborato\u0159\u00ED a v t\u00E9to pr\u00E1ci je pops\u00E1na metoda, jak lze \u00FAsp\u011B\u0161n\u011B i hmotnostn\u00ED spektrometrii vyu\u017E\u00EDt p\u0159i anal\u00FDze chir\u00E1ln\u00EDch l\u00E1tek. Mezi nejperspektivn\u011Bj\u0161\u00ED metody pat\u0159\u00ED Cooksova kinetick\u00E1 metoda. C\u00EDlem t\u00E9to pr\u00E1ce je modifikovat tuto metodu tak, aby umo\u017E\u0148ovala anal\u00FDzu chir\u00E1ln\u00EDch l\u00E1tek i v pr\u016Fto\u010Dn\u00FDch syst\u00E9mech, \u010D\u00EDm\u017E lze dos\u00E1hnout vy\u0161\u0161\u00ED efektivity p\u0159i vlastn\u00ED anal\u00FDze. Pomoc\u00ED autosampleru je d\u00E1vkov\u00E1n analyt A (sm\u011Bs enantiomer\u016F aminokyselin) do proudu nosn\u00E9 kapaliny (voda-methanol; 1:1; v/v). P\u0159ed vstupem do iontov\u00E9ho zdroje (elektrospej) je k nosn\u00E9 kapalin\u011B kontinu\u00E1ln\u011B p\u0159iv\u00E1d\u011Bn roztok s referen\u010Dn\u00ED l\u00E1tkou ref* (optick\u00FD izomer) a"@cs . "Fry\u010D\u00E1k, Petr" . . . . "Anal\u00FDza chir\u00E1ln\u00EDch l\u00E1tek pat\u0159\u00ED vzhledem ke sv\u00E9mu v\u00FDznamu pro oborz bezprost\u0159edn\u011B ovliv\u0148uj\u00EDc\u00ED kvalitu lidsk\u00E9ho \u017Eivota (nap\u0159. v\u00FDvoj a v\u00FDroba l\u00E9\u010Div) k d\u016Fle\u017Eit\u00FDm a st\u00E1le se rozv\u00EDjej\u00EDc\u00EDm odv\u011Btv\u00EDm analytick\u00E9 chemie. Mezi rutinn\u011B pou\u017E\u00EDvan\u00E9 metody v t\u00E9to oblasti pat\u0159\u00ED zejm\u00E9na vysoko\u00FA\u010Dinn\u00E1 kapalinov\u00E1 chromatografie a kapil\u00E1rn\u00ED elektrofor\u00E9za. Hmotnostn\u00ED spektrometrie se st\u00E1v\u00E1 b\u011B\u017Enou sou\u010D\u00E1st\u00ED mnoha laborato\u0159\u00ED a v t\u00E9to pr\u00E1ci je pops\u00E1na metoda, jak lze \u00FAsp\u011B\u0161n\u011B i hmotnostn\u00ED spektrometrii vyu\u017E\u00EDt p\u0159i anal\u00FDze chir\u00E1ln\u00EDch l\u00E1tek. Mezi nejperspektivn\u011Bj\u0161\u00ED metody pat\u0159\u00ED Cooksova kinetick\u00E1 metoda. C\u00EDlem t\u00E9to pr\u00E1ce je modifikovat tuto metodu tak, aby umo\u017E\u0148ovala anal\u00FDzu chir\u00E1ln\u00EDch l\u00E1tek i v pr\u016Fto\u010Dn\u00FDch syst\u00E9mech, \u010D\u00EDm\u017E lze dos\u00E1hnout vy\u0161\u0161\u00ED efektivity p\u0159i vlastn\u00ED anal\u00FDze. Pomoc\u00ED autosampleru je d\u00E1vkov\u00E1n analyt A (sm\u011Bs enantiomer\u016F aminokyselin) do proudu nosn\u00E9 kapaliny (voda-methanol; 1:1; v/v). P\u0159ed vstupem do iontov\u00E9ho zdroje (elektrospej) je k nosn\u00E9 kapalin\u011B kontinu\u00E1ln\u011B p\u0159iv\u00E1d\u011Bn roztok s referen\u010Dn\u00ED l\u00E1tkou ref* (optick\u00FD izomer) a" . . "Rozli\u0161en\u00ED izomer\u016F hmotnostn\u00ED spektrometri\u00ED s vyu\u017Eit\u00EDm kinetick\u00E9 metody" . "15310" . "CZ - \u010Cesk\u00E1 republika" . . . "Rozli\u0161en\u00ED izomer\u016F hmotnostn\u00ED spektrometri\u00ED s vyu\u017Eit\u00EDm kinetick\u00E9 metody"@cs . "Ranc, V\u00E1clav" . . "M\u00FCller, Luk\u00E1\u0161" . . . . "[B002FB431391]" . . "Recognition of isomers by mass spectrometry using the kinetic method"@en . . "Rozli\u0161en\u00ED izomer\u016F hmotnostn\u00ED spektrometri\u00ED s vyu\u017Eit\u00EDm kinetick\u00E9 metody" . "3" . "Lemr, Karel" . . "100" . "Bedn\u00E1\u0159, Petr" . "Z(MSM6198959216)" . "Chemick\u00E9 listy" . . "mass spectrometry; kinetic method; isomer recognition"@en . "The principle of the Cooks'kinetic method and its application to analysis of isomeric mixtures are described. The kinetic method based on mass spectrometric measurements is applied to discrimination of isomers of various types of compounds (amino acids, peptides, sugars, pharmaceuticals, etc.). So far this method has been used in static systems (with constant analyte concentrations in the course of analytical run). A modification for dynamic systems (flow injection analysis, HPLC) is presented. The accuracy of determination in both static and dynamic systems can worsen due to different ratios of the isomers in liquid and gas phases. A procedure for correction of this phenomenon is also demonstrated."@en . "Recognition of isomers by mass spectrometry using the kinetic method"@en . "8"^^ .