. "P(ME 664), Z(MSM6198959216)" . . . "Fr\u00E9bort, Ivo" . . . . . . "Aspergillus niger; mass specrometry; methylamine oxidase; peptide; primary structure"@en . "5" . "3"^^ . "0015-5632" . "RIV/61989592:15310/05:00002191" . . . "Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger" . "8"^^ . "[53EF209E25FB]" . "Lenobel, Ren\u00E9" . . "Mapov\u00E1n\u00ED prim\u00E1rn\u00ED struktury methylaminoxidasy z Aspergillus niger obsahuj\u00EDc\u00ED topachinon a m\u011B\u010F"@cs . "3"^^ . "The amino acid sequence of methylamine oxidase (MeAO) from the fungus Aspergillus niger was analyzed using mass spectrometry (MS). First, MeAO was characterized by an accurate molecular mass of 72.4 kDa of the monomer measured using MALDI-TOF-MS and by a pI value of 5.8 determined by isoelectric focusing. MALDI-TOF-MS revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from that of A. niger amine oxidase AO-I. Tandem mass spectrometry with electrospray ionization coupled to liquid chromatography allowed unambiguous reading of six peptide sequences (11-19 amino acids) and seven sequence tags (4-15 amino acids), which were used for MS BLAST homology searching. MeAO was found to be largely homologous to a hypothetical protein AN7641.2 (EMBL/GenBank protein accession code EAA61827) from Aspergillus nidulans FGSC A4 with a theoretical molecular mass of 76 460 Da and pI 6.14, which b"@en . . "Mapov\u00E1n\u00ED prim\u00E1rn\u00ED struktury methylaminoxidasy z Aspergillus niger obsahuj\u00EDc\u00ED topachinon a m\u011B\u010F"@cs . "Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger" . . . "The amino acid sequence of methylamine oxidase (MeAO) from the fungus Aspergillus niger was analyzed using mass spectrometry (MS). First, MeAO was characterized by an accurate molecular mass of 72.4 kDa of the monomer measured using MALDI-TOF-MS and by a pI value of 5.8 determined by isoelectric focusing. MALDI-TOF-MS revealed a clear peptide mass fingerprint after tryptic digestion, which did not provide any relevant hit when searched against a nonredundant protein database and was different from that of A. niger amine oxidase AO-I. Tandem mass spectrometry with electrospray ionization coupled to liquid chromatography allowed unambiguous reading of six peptide sequences (11-19 amino acids) and seven sequence tags (4-15 amino acids), which were used for MS BLAST homology searching. MeAO was found to be largely homologous to a hypothetical protein AN7641.2 (EMBL/GenBank protein accession code EAA61827) from Aspergillus nidulans FGSC A4 with a theoretical molecular mass of 76 460 Da and pI 6.14, which b" . "Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger"@en . "RIV/61989592:15310/05:00002191!RIV06-MSM-15310___" . "15310" . . "CZ - \u010Cesk\u00E1 republika" . "Aminokyselinov\u00E1 sekvence methylaminoxidasy izolovan\u00E9 z pl\u00EDsn\u011B Aspergillus niger byla analyzov\u00E1na r\u016Fzn\u00FDmi MS technikami. MALDI-MS umo\u017Enila stanoven\u00ED p\u0159esn\u00E9 hmoty monomeru o velikosti 72,4 kDa. Isoelektrick\u00FD bod byl ur\u010Den pomoc\u00ED isoelektrick\u00E9 fokusace. Peptidov\u00E9 mapov\u00E1n\u00ED pomoc\u00ED MALDI-MS uk\u00E1zalo, \u017Ee sekvence methylaminoxidasy se v\u00FDrazn\u011B li\u0161\u00ED od sekvence aminoxidasy AO-I. \u010C\u00E1ste\u010Dn\u00E1 sekvence vyizolovan\u00E9 methylaminoxidasy byla ur\u010Dena pomoc\u00ED konbinace p\u0159\u00EDstupu MALDI-PSD-MS a nanoLC-ESI-MSMS a s vyu\u017Eit\u00EDm MS BLAST softwaru pro hled\u00E1n\u00ED homologie podle zadan\u00FDch peptidov\u00FDch sekvenc\u00ED proteinu. V datab\u00E1zi byl nalezen homologn\u00ED hypotetick\u00FD protein AN7641.2 (EMBL/GenBank protein-accession code EAA61827) z Aspergillus nidulans FGSC A4 o teoretick\u00E9 molekulov\u00E9 hmotnosti 76.46 kDa a pI 6.14, ktery pat\u0159\u00ED do skupiny aminoxidas obsahuj\u00EDc\u00EDch m\u011B\u010F. Tento protein vykazuje pouze malou homologii k aminooxidase AO-I (32 % identity, 49 % podobnost)."@cs . "\u0160ebela, Marek" . "50" . . "401-408" . "Folia Microbiologica" . . "Mapping the primary structure of copper/topaquinone-containing methylamine oxidase from Aspergillus niger"@en . . . . "528868" . .