. "RIV/61989592:15310/05:00001951!RIV11-MSM-15310___" . "batch immunoextraction; 6-benzylaminopurine; antibody"@en . . "P(IBS4055304), S, Z(AV0Z50380511), Z(MSM6198959216)" . . . "10"^^ . . "Hauserov\u00E1, Eva" . "Fuksov\u00E1, Kv\u011Btoslava" . "Batch immunoextraction method for efficient purification of aromatic cytokinins" . . . "RIV/61989592:15310/05:00001951" . . "Hajd\u00FAch, Mari\u00E1n" . . "Journal of Chromatography A: Symposium Volumes" . "000234158600015" . "Strnad, Miroslav" . . "Lenobel, Ren\u00E9" . . . . "Batch immunoextraction method for efficient purification of aromatic cytokinins"@en . "[A61D8E5B5FAC]" . "6"^^ . "1100" . "513576" . "Dole\u017Eal, Karel" . . "A range of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In order to study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complex samples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody had undetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as some synthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificity in enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificity and capacity" . "9"^^ . . "0021-9673" . "NL - Nizozemsko" . . "Swaczynov\u00E1, Jana" . . "Popa, Igor" . . . "Vydra, David" . "A range of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under in vitro conditions. In order to study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complex samples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody had undetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as some synthetic di- and tri-substituted 6-benzylaminopurines and the corresponding ribosides. The antibody also showed strong responses and specificity in enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificity and capacity"@en . . "Batch immunoextraction method for efficient purification of aromatic cytokinins"@en . "1" . . . . "Batch immunoextraction method for efficient purification of aromatic cytokinins" . . "15310" .