"Canberra" . . "144"^^ . "[D8D318B9C36A]" . "New methodological approaches to plant hormone analysis"@en . "4"^^ . . . . . . "Jsou uvedeny nov\u00E9 metohologick\u00E9 p\u0159\u00EDstupy v anal\u00FDz\u00E1ch rostlinn\u00FDch hormon\u016F."@cs . "P(GA522/01/0275)" . "576122" . "Nov\u00E9 metohologick\u00E9 p\u0159\u00EDstupy v anal\u00FDz\u00E1ch rostlinn\u00FDch hormon\u016F"@cs . "immunoaffinity chromatography;mass spectrometry;cytokinin;plant hormones"@en . "New methodological approaches to plant hormone analysis" . "Australian National University" . "Dole\u017Eal, Karel" . . . "Nov\u00E1k, Ond\u0159ej" . "New approach in the analytical technologies of plant hormone analysis was introduced in the field of cytokinins and might be also soon applied in the analysis of other plant hormone groups. A new sensitive method for the quantitative analysis of all natural isoprenoid and aromatic cytokinins in plants is based on combined %22generic%22 immunoaffinity chromatography - electrospray single-quadrupole mass. A baseline chromatographic separation of 20 non-derivatized naturally occurring cytokinins has been obtained. Precise analysis of O-glucoside and nucleotide fractions were also performed by the HPLC-MS runs but after an enzymatic treatment. More than 50 different cytokinin metabolites can be measured by this approach. When low cone voltage (20 eV) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of M+H + with limits of detection from 10 to 50 fmol. For routine analysis we obtained a linearity range between 25 (75) fmol - 100 pmol. Developed LC-MS"@en . . "Sp\u00EDchal, Luk\u00E1\u0161" . . "New approach in the analytical technologies of plant hormone analysis was introduced in the field of cytokinins and might be also soon applied in the analysis of other plant hormone groups. A new sensitive method for the quantitative analysis of all natural isoprenoid and aromatic cytokinins in plants is based on combined %22generic%22 immunoaffinity chromatography - electrospray single-quadrupole mass. A baseline chromatographic separation of 20 non-derivatized naturally occurring cytokinins has been obtained. Precise analysis of O-glucoside and nucleotide fractions were also performed by the HPLC-MS runs but after an enzymatic treatment. More than 50 different cytokinin metabolites can be measured by this approach. When low cone voltage (20 eV) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of M+H + with limits of detection from 10 to 50 fmol. For routine analysis we obtained a linearity range between 25 (75) fmol - 100 pmol. Developed LC-MS" . "Nov\u00E9 metohologick\u00E9 p\u0159\u00EDstupy v anal\u00FDz\u00E1ch rostlinn\u00FDch hormon\u016F"@cs . "15310" . . "Lenobel, Ren\u00E9" . "RIV/61989592:15310/04:00002446" . . . . "Strnad, Miroslav" . . . "RIV/61989592:15310/04:00002446!RIV/2005/GA0/153105/N" . . "87" . "5"^^ . . . "18th IPGSA conference, Conference Handbook" . "New methodological approaches to plant hormone analysis" . "New methodological approaches to plant hormone analysis"@en .