. "-" . . "PL - Polsk\u00E1 republika" . . . "Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues"@en . . "Brassinosteroids (BRs) represent a large group of plant steroids which include more than 40 congeners distributed from lower to higher plants. BRs have been detected and isolated from seeds, fruits, leaves, galls and pollen. Physiological functions proposed for BRs include cell elongation, cell division and modulation of stress responses.We have developed polyclonal antibodies against a brassinosteroid analogue, (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid (4812). Antiserum against this substance was raised in rabbits immunized using 4812 - bovine-serum albumin (BSA) conjugate. The conjugate was prepared by mixed anhydride method.Polyclonal antibodies raised in rabbits were purified by ammonium sulphate precipitation or by affinity purification on protein A columns. The obtained antibodies were tested in enzyme-linked immunosorbent assay (ELISA) using (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid - horse-radish peroxidase conjugate.The cross" . . "Swaczynov\u00E1, Jana" . "Kohout, Ladislav" . . "0137-5881" . "Brassinosteroids (BRs) represent a large group of plant steroids which include more than 40 congeners distributed from lower to higher plants. BRs have been detected and isolated from seeds, fruits, leaves, galls and pollen. Physiological functions proposed for BRs include cell elongation, cell division and modulation of stress responses.We have developed polyclonal antibodies against a brassinosteroid analogue, (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid (4812). Antiserum against this substance was raised in rabbits immunized using 4812 - bovine-serum albumin (BSA) conjugate. The conjugate was prepared by mixed anhydride method.Polyclonal antibodies raised in rabbits were purified by ammonium sulphate precipitation or by affinity purification on protein A columns. The obtained antibodies were tested in enzyme-linked immunosorbent assay (ELISA) using (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid - horse-radish peroxidase conjugate.The cross"@en . "3"^^ . . . "Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues" . "Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues" . "Byla studov\u00E1na adaptace enzymov\u00E9 imunoanal\u00FDzy pro stanoven\u00ED brassinosteroid\u016F v rostlinn\u00E9m materi\u00E1lu."@cs . "318"^^ . "26" . "2"^^ . "28" . "[4F8E42086ECE]" . "Adaptace enzymov\u00E9 imunoanal\u00FDzy pro stanoven\u00ED brassinosteroid\u016F v rostlinn\u00E9m materi\u00E1lu"@cs . . "Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues"@en . . . . "Acta Physiologiae Plantarum" . "RIV/61989592:15310/04:00002318" . "P(IBS5038351)" . . "brassinosteroids;polyclonal antibodies;ELISA"@en . . "RIV/61989592:15310/04:00002318!RIV/2005/AV0/153105/N" . "15310" . "Strnad, Miroslav" . "Adaptace enzymov\u00E9 imunoanal\u00FDzy pro stanoven\u00ED brassinosteroid\u016F v rostlinn\u00E9m materi\u00E1lu"@cs . . "553506" .