"18th IPGSA conference, Conference Handbook" . "Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Nov\u00E1k et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and collision energy fo" . "Nov\u00E1k, Ond\u0159ej" . "Application of Q-TOF technology in plant hormone research" . "Application of Q-TOF technology in plant hormone research"@en . . . . "Canberra" . "Australian National University" . . "Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Nov\u00E1k et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and collision energy fo"@en . "P(GA522/03/0323), Z(MSM 153100008)" . . . "RIV/61989592:15310/04:00002217!RIV/2005/GA0/153105/N" . "Richer, Stephanie" . "144"^^ . . "15310" . . . . . "RIV/61989592:15310/04:00002217" . . . "124" . . . "liquid chromatography;mass spectrometry;quadrupole-time of flight mass spectrometer;cytokinins;identification;quantification;exact mass determination"@en . . "Kombinace HPLC nebo kapil\u00E1rn\u00ED kapalinov\u00E9 chromatografie (CapLC) s Q-TOF d\u00E1v\u00E1 nov\u00E9 mo\u017Enosti ve v\u00FDzkumu rostlinn\u00FDch hormon\u016F."@cs . "555158" . "Strnad, Miroslav" . "Pou\u017Eit\u00ED Q-TOF technologie p\u0159i studiu rostlinn\u00FDch hormon\u016F"@cs . . . . "Application of Q-TOF technology in plant hormone research"@en . "[84106E3138A2]" . . "3"^^ . . "Pou\u017Eit\u00ED Q-TOF technologie p\u0159i studiu rostlinn\u00FDch hormon\u016F"@cs . "Application of Q-TOF technology in plant hormone research" . "4"^^ . . "Lenobel, Ren\u00E9" . .