. "Analytica Chimica Acta" . "Kvantitavn\u00ED stanoven\u00ED cytokinin\u016F v rostlin\u00E1ch metodou kapalinov\u00E9 chromatografie-hmotnostn\u00ED spektrometrie"@cs . . . "2" . . "0003-2670" . "Pro stanoven\u00ED v\u011Bt\u0161iny p\u0159irozen\u011B se vyskytuj\u00EDc\u00EDch isoprenoidn\u00EDch a aromatick\u00FDch cytokinin\u016F v rostlin\u00E1ch jsme vyvinuli novou citlivou metodu. Jej\u00EDm z\u00E1kladem je chromatografick\u00E1 separace 20 rostlinn\u00FDch hormon\u016F - cytokinin\u016F (b\u00E1ze, ribosidy a 9-glukosidy) na reverzn\u00ED f\u00E1zi ve spojen\u00ED s hmotnostn\u00ED detekc\u00ED na jednoduch\u00E9m kvadrup\u00F3lu (HPLC/MS), za podm\u00EDnek ionizace elektrosprejem. Pro stanoven\u00ED O-glukosid\u016F a nukleotid\u016F cytokinin\u016F lze tuto metodu rovn\u011B\u017E s \u00FAsp\u011Bchem pou\u017E\u00EDt po p\u0159edchoz\u00EDm enzymov\u00E9m o\u0161et\u0159en\u00ED rostlinn\u00FDch extrakt\u016F. Spolehlivost metody byla validov\u00E1na za pomoci sm\u011Bsi deuterovan\u00FDch cytokininov\u00FDch standard\u016F p\u0159id\u00E1van\u00FDch p\u0159i extrakci rostlinn\u00E9ho materi\u00E1lu. Rostlinn\u00FD materi\u00E1l byl po extrakci do Bielesk\u00E9ho roztoku p\u0159e\u010Di\u0161t\u011Bn pou\u017Eit\u00EDm dvojn\u00E1sobn\u00E9 ionexov\u00E9 chromatografie (SCX a DEAE Sephadexu) v kombinaci s imunoafinitn\u00ED chromatografi\u00ED na gelu s nav\u00E1zan\u00FDmi skupinov\u011B selektivn\u00EDmi monoklon\u00E1ln\u00EDmi protil\u00E1tkami proti cytokinin\u016Fm. P\u0159\u00EDtomnost isoprenoidn\u00EDch a aromatick\u00FDch cytokinin\u016F ve studovan\u00FDch extraktech byla sou\u010Da"@cs . "Kvantitavn\u00ED stanoven\u00ED cytokinin\u016F v rostlin\u00E1ch metodou kapalinov\u00E9 chromatografie-hmotnostn\u00ED spektrometrie"@cs . "RIV/61989592:15310/03:00002990" . "Lenobel, Ren\u00E9" . . . . "Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry"@en . . "NL - Nizozemsko" . . . "12"^^ . . "Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry" . . "RIV/61989592:15310/03:00002990!RIV07-GA0-15310___" . "Tarkowski, Petr" . "207-218" . . "[BD4466B15F9E]" . . . "Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry" . "A sensitive method for the quantitative analysis of all natural isoprenoid cytokinins in plant material by electrospray single-quadrupole mass spectrometry is presented. A baseline chromatographic separation of 20 non-derivatised naturally occurring cytokinins has been developed. Precise analyses of O-glucoside and ribonucleotide fractions were also performed by the high-performance liquid chromatography-mass spectrometry (HPLC-MS) but run separately from the basic cytokinin metabolites. Using post-column splitting, the flux from narrow-bore (2.1 mm, i.d.) reversed-phase liquid chromatography column was simultaneously introduced into the diode array and mass detector. Optimal conditions, including final flow rate, desolvation temperature, desolvation gas flow, capillary and cone voltage for effective ionisation in the electrospray ion source were found. When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M + H](+) wi" . . "P(GA522/01/0275), P(GA522/03/0323), Z(AV0Z5038910), Z(MSM 153100008)" . "Dole\u017Eal, Karel" . . "Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry"@en . "480" . "P\u011Bchov\u00E1, Danu\u0161e" . "6"^^ . . "Strnad, Miroslav" . . "6"^^ . "liquid chromatography; mass spectrometry; cytokinins; ELISA; tobacco; poplar; tissue; 6-benzylaminopurine; quantification; derivatives; metabolism"@en . . . . . . . . . . . "Nov\u00E1k, Ond\u0159ej" . . "624387" . "15310" . . . "A sensitive method for the quantitative analysis of all natural isoprenoid cytokinins in plant material by electrospray single-quadrupole mass spectrometry is presented. A baseline chromatographic separation of 20 non-derivatised naturally occurring cytokinins has been developed. Precise analyses of O-glucoside and ribonucleotide fractions were also performed by the high-performance liquid chromatography-mass spectrometry (HPLC-MS) but run separately from the basic cytokinin metabolites. Using post-column splitting, the flux from narrow-bore (2.1 mm, i.d.) reversed-phase liquid chromatography column was simultaneously introduced into the diode array and mass detector. Optimal conditions, including final flow rate, desolvation temperature, desolvation gas flow, capillary and cone voltage for effective ionisation in the electrospray ion source were found. When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M + H](+) wi"@en .