. "The development of biosensor for the determination of amylase activity"@en . "Zajoncov\u00E1, Ludmila" . "642861" . . . . . "410"^^ . "amylase; biosensor; activity determination"@en . . . "J\u00EDlek, Milan" . . "Pe\u010D, Pavel" . "15310" . . "[51276A2C91ED]" . "Slovensk\u00E1 spolo\u010Dnos\u0165 pre bioch\u00E9miu a molekul\u00E1rnu biol\u00F3giu pri SAV" . . "The development of biosensor for the determination of amylase activity"@en . "0"^^ . "4"^^ . "The development of biosensor for the determination of amylase activity" . "0"^^ . "The development of biosensor for the determination of amylase activity" . "Star\u00E1 Lesn\u00E1" . . "a-Amylase (EC 3.2.1.1) catalyze the hydrolysis of starch to a significant extent resulting in the release of maltose. Determination of serum a-amylase activity is a procedure widely used, for example, in clinical medicine for the diagnosis of acute pancreatitis. The procedures most frequently used to determine a-amylase activity are either amyloclastic or saccharogenic (MAZZUCHIN et al., 1973). The former is based on the starch-iodine reaction and the latter on the formation of reducing sugars, measuredas maltose or glucose equivalents. Biosensor determination of maltose is based on an oxygen Clark type electrode equipped with a suitable enzymatic membrane (FILIPIAK et al.,1996). This membrane can be obtained by immobilization of the enzymes hydrolysi ng maltose (a-glucosidase) to glucose, which is determined using an oxygen electrode with the glucose oxidase membrane. The new method of determination of ?-amylase activity is based on determination of produced maltose using the Clark electrode or hydr" . "a-Amylase (EC 3.2.1.1) catalyze the hydrolysis of starch to a significant extent resulting in the release of maltose. Determination of serum a-amylase activity is a procedure widely used, for example, in clinical medicine for the diagnosis of acute pancreatitis. The procedures most frequently used to determine a-amylase activity are either amyloclastic or saccharogenic (MAZZUCHIN et al., 1973). The former is based on the starch-iodine reaction and the latter on the formation of reducing sugars, measuredas maltose or glucose equivalents. Biosensor determination of maltose is based on an oxygen Clark type electrode equipped with a suitable enzymatic membrane (FILIPIAK et al.,1996). This membrane can be obtained by immobilization of the enzymes hydrolysi ng maltose (a-glucosidase) to glucose, which is determined using an oxygen electrode with the glucose oxidase membrane. The new method of determination of ?-amylase activity is based on determination of produced maltose using the Clark electrode or hydr"@en . "Bratislava" . "18th Joint Congress of the Czech and Slovak Societies for Biochemistry and Molecular Biology" . "2"^^ . "RIV/61989592:15310/02:00001687!RIV/2003/MSM/153103/N" . . . . . "RIV/61989592:15310/02:00001687" . "Beranov\u00E1, Veronika" . "Z(MSM 153100010)" . . "148" . .