. "RIV/61989592:15310/02:00001609" . . . "\u0160ebela, Marek" . "642843" . "Plant copper amine oxidases (CuAOS; EC 1. 4. 3. 6) belong to the quinoprotein family. Besides the cofactor topaquinone, they also contain cupric ions participating in the reaction. They catalyse the oxidative deamination of amine substrates to the respective aldehydes, ammonia and hydrogen peroxide (MEDDA, 1995). We studied interactions of pea seedling enzyme (PSAO) with several artifical amine compounds that have not yet been analysed in this way and whose structure mimic natural polyamine substrates. In the second part we focused our attention on the interaction of optically active sedamine alkaloids and various pyridine-derived oximes with PSAO. 3-Oxapentane-1,5-diamine (OPD), 1, 4-bis-(3-aminopropyl)piperazine and N, N-bis(3-aminopropyl)-trans-2-butene were found to be substrates of PSAO. The corresponding enzyme kinetics was measured, the reaction stechiometry analysed and rapid scanning spectrophotometry applied to investigate the reaction mechanism. A similar compound to OPD, 3-azapentane-1,5-d" . . "Development in the course of study substrates and inhibitors of plant copper amine oxidases"@en . . "0"^^ . "0"^^ . "Plant copper amine oxidases (CuAOS; EC 1. 4. 3. 6) belong to the quinoprotein family. Besides the cofactor topaquinone, they also contain cupric ions participating in the reaction. They catalyse the oxidative deamination of amine substrates to the respective aldehydes, ammonia and hydrogen peroxide (MEDDA, 1995). We studied interactions of pea seedling enzyme (PSAO) with several artifical amine compounds that have not yet been analysed in this way and whose structure mimic natural polyamine substrates. In the second part we focused our attention on the interaction of optically active sedamine alkaloids and various pyridine-derived oximes with PSAO. 3-Oxapentane-1,5-diamine (OPD), 1, 4-bis-(3-aminopropyl)piperazine and N, N-bis(3-aminopropyl)-trans-2-butene were found to be substrates of PSAO. The corresponding enzyme kinetics was measured, the reaction stechiometry analysed and rapid scanning spectrophotometry applied to investigate the reaction mechanism. A similar compound to OPD, 3-azapentane-1,5-d"@en . . . . . "4"^^ . "110" . . "Development in the course of study substrates and inhibitors of plant copper amine oxidases" . "amine; amine oxidase; enzyme; inhibitor; substrate"@en . "Pe\u010D, Pavel" . . . . "Ad\u00E1mkov\u00E1, \u0160\u00E1rka" . "[DE45D5EBA304]" . . . "Slovensk\u00E1 spolo\u010Dnos\u0165 pre bioch\u00E9miu a molekul\u00E1rnu biol\u00F3giu pri SAV" . "Ml\u00ED\u010Dkov\u00E1, Kate\u0159ina" . "8"^^ . "Pet\u0159ivalsk\u00FD, Marek" . . "Z(MSM 153100010)" . . "Development in the course of study substrates and inhibitors of plant copper amine oxidases"@en . . "Development in the course of study substrates and inhibitors of plant copper amine oxidases" . "Star\u00E1 Lesn\u00E1" . "RIV/61989592:15310/02:00001609!RIV/2003/MSM/153103/N" . "Fr\u00E9bort, Ivo" . . "Lamplot, Zbyn\u011Bk" . "Bratislava" . . "Lemr, Karel" . "18th Joint Congress of the Czech and Slovak Societies for Biochemistry and Molecular Biology" . "410"^^ . "15310" . .