. "Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris"@en . "80-7080-437-8" . "15310" . "6"^^ . "P(GA203/00/D119)" . "RIV/61989592:15310/01:00002026!RIV/2005/GA0/153105/N" . . "Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris" . . . . "Zajoncov\u00E1, Ludmila" . "6"^^ . "amine oxidase;enzyme;inhibitor;Phaseolus vulgaris"@en . . . . . "Lemr, Karel" . "Proceedings of 2nd International Symposium %22Separations in the BioSciences - SBS 2001%22" . "\u010Cesk\u00E1 spole\u010Dnost chemick\u00E1" . . . "Luhov\u00E1, Lenka" . "Praha" . "Pet\u0159ivalsk\u00FD, Marek" . . . "Byla provedena purifikace a charakterizace aminoxidasy a jej\u00EDho endogenn\u00EDho inhibitoru z Phaseolus vulgaris."@cs . "Pe\u010D, Pavel" . . "[DC4556D7642F]" . . "Amine oxidase activity in the seedlings homogenates of common bean (Phaseolus vulgaris) was reported to be absent due to the presence of low-molecular endogenous inhibitor. We investigated the changes in amine oxidase activity in developing bean seedlings by the use of biosensor-immobilized tissue cuttings and by histochemical analysis. Amine oxidase was isolated and purified to apparent homogeneity from 5-days old etiolated seedlings. The isolation scheme included fractionation by ammonium sulphate, ion-exchange chromatography on DEAE-cellulose and hydrophobic chromatography on hydroxyapatite. Further purification was achieved through HPLC on ion-exchange and gel columns. Purified enzyme was characterised by native and SDS-electrophoresis. After native electrophoresis, the enzyme was transferred to cellulose acetate membranes by semi-dry blotting, but antibodies to amine oxidase from pea (Pisum sativum) did not show cross-reactivity with corresponding protein from Phaseolus. Spectroscopic properties"@en . "Purifikace a charakterizace aminoxidasy a jej\u00EDho endogenn\u00EDho inhibitoru z Phaseolus vulgaris."@cs . "RIV/61989592:15310/01:00002026" . "693707" . . "\u0160ebela, Marek" . "Amine oxidase activity in the seedlings homogenates of common bean (Phaseolus vulgaris) was reported to be absent due to the presence of low-molecular endogenous inhibitor. We investigated the changes in amine oxidase activity in developing bean seedlings by the use of biosensor-immobilized tissue cuttings and by histochemical analysis. Amine oxidase was isolated and purified to apparent homogeneity from 5-days old etiolated seedlings. The isolation scheme included fractionation by ammonium sulphate, ion-exchange chromatography on DEAE-cellulose and hydrophobic chromatography on hydroxyapatite. Further purification was achieved through HPLC on ion-exchange and gel columns. Purified enzyme was characterised by native and SDS-electrophoresis. After native electrophoresis, the enzyme was transferred to cellulose acetate membranes by semi-dry blotting, but antibodies to amine oxidase from pea (Pisum sativum) did not show cross-reactivity with corresponding protein from Phaseolus. Spectroscopic properties" . . "122" . "Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris"@en . . . "159"^^ . . . "Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris" . "Purifikace a charakterizace aminoxidasy a jej\u00EDho endogenn\u00EDho inhibitoru z Phaseolus vulgaris."@cs .