. . . "Studie reakc\u00ED rostlinn\u00E9 Cu aminooxidasy s C3 a C4 alifatick\u00FDmi diaminy"@cs . "Pe\u010D, Pavel" . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Studie reakc\u00ED rostlinn\u00E9 Cu aminooxidasy s C3 a C4 alifatick\u00FDmi diaminy"@cs . "384" . . . "0003-9861" . . . "1" . "\u0160ebela, Marek" . . . . . . . . "Lemr, Karel" . . "Archives of Biochemistry and Biophysics" . . "12"^^ . "[37F43E6B30BD]" . "Fr\u00E9bort, Ivo" . . "4"^^ . . . "RIV/61989592:15310/00:00007755" . "P(GA203/97/0097), P(GA203/99/D048), P(ME 153), Z(MSM 153100010), Z(MSM 153100013)" . "RIV/61989592:15310/00:00007755!RIV09-MSM-15310___" . . "\u010Cl\u00E1nek se zab\u00FDv\u00E1 studiem reakce aminoxidasy z hrachoru set\u00E9ho (Lathyrus sativus) s n\u011Bkolika alifatick\u00FDmi diaminy. Byl zji\u0161\u0165ov\u00E1n vliv d\u00E9lky uhl\u00EDkov\u00E9ho \u0159et\u011Bzce a p\u0159\u00EDtomnost nenasycen\u00FDch vazeb na reaktivitu. Trans- tj. (E)-2-buten-1,4-diamin (TDABE) a cis- tj. (Z)-2-buten-1,4-diamin (CDABE) byly dobr\u00FDmi substr\u00E1ty enzymu. Propan-1,3-diamin (DAP) a propen-1,3-diamin (DAPE) byly pouze slab\u011B oxidov\u00E1ny, zat\u00EDmco 1,3-diamino-2-propanol (DAPL) nebyl jako substr\u00E1t v\u016Fbec vyu\u017E\u00EDv\u00E1n. Enzym byl irreverzibiln\u011B inhibov\u00E1n l\u00E1tkou DABI (2-butyn-1,4-diamin). Pr\u016Fb\u011Bh reakc\u00ED, v\u010Detn\u011B identifikace meziprodukt\u016F, byl analyzov\u00E1n spektroskopicky, n\u011Bkter\u00E9 reak\u010Dn\u00ED produkty byly ov\u011B\u0159eny pomoc\u00ED hmotnostn\u00ED spektrometrie."@cs . "The paper reports a study on the reactions of grass pea (Lathyrus sativus) amine oxidase (GPAO) with several aliphatic diamines. The influence of the chain length and of unsaturations in the molecules was examined. Kinetic measurements confirmed that trans-, i.e., (E)-2-butene-1,4 diamine (TDABE) and cis-, i.e., (Z)-2-butene-1,4-diamine (CDABE) could be classified as good substrates. Propane-1,3 diamine (DAP) and propene-1,3-diamine (DAPE) were only weakly oxidized, whereas 1,3-diamino-2-propanol (DAPL) was not utilized as a substrate. Contrary to the inactivator 2-butyne 1,4-diamine (DABDI), DAPE was shown to be only a competitive inhibitor. DAP itself did not inhibit the catalytic activity, irreversible inhibition of the activity occurred only after the incubation of GPAO with DABI; other diamines were without this effect. Differential pulse polarography and chromatofocusing confirmed that the aminoaldehyde product of DABI oxidation binds to the enzyme. Activity assay of pea aminoaldehyde dehydrogen"@en . "703741" . "A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines" . . . . . . . "Brauner, F." . "The paper reports a study on the reactions of grass pea (Lathyrus sativus) amine oxidase (GPAO) with several aliphatic diamines. The influence of the chain length and of unsaturations in the molecules was examined. Kinetic measurements confirmed that trans-, i.e., (E)-2-butene-1,4 diamine (TDABE) and cis-, i.e., (Z)-2-butene-1,4-diamine (CDABE) could be classified as good substrates. Propane-1,3 diamine (DAP) and propene-1,3-diamine (DAPE) were only weakly oxidized, whereas 1,3-diamino-2-propanol (DAPL) was not utilized as a substrate. Contrary to the inactivator 2-butyne 1,4-diamine (DABDI), DAPE was shown to be only a competitive inhibitor. DAP itself did not inhibit the catalytic activity, irreversible inhibition of the activity occurred only after the incubation of GPAO with DABI; other diamines were without this effect. Differential pulse polarography and chromatofocusing confirmed that the aminoaldehyde product of DABI oxidation binds to the enzyme. Activity assay of pea aminoaldehyde dehydrogen" . "5"^^ . . . "15310" . "A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines"@en . "CRYSTAL-STRUCTURE; MECHANISM; PEA; ENZYME; 1; 4-DIAMINO-2 BUTENE; INHIBITORS; RESOLUTION; SUBSTRATE; OXIDATION; SEEDLINGS"@en . "A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines"@en . . "A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines" . . .