. "A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA"@en . . . "A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA" . . "3"^^ . . "RIV/61989592:15110/13:33143922!RIV15-TA0-15110___" . . "copper(I) ions; light microscopy; mitochondrial DNA; nuclear DNA"@en . "10.1371/journal.pone.0066864" . . . . "P(ED0030/01/01), P(TA03010598), P(TA03010719)" . "2"^^ . "6" . "The study describes the method of a sensitive detection of double-stranded DNA molecules in situ. It is based on the oxidative attack on the deoxyribose moiety by copper(I) in the presence of oxygen. We have shown previously that the oxidative attack leads to the formation of frequent gaps in DNA. Here we have demonstrated that the gaps can be utilized as the origins for an efficient synthesis of complementary labeled strands by DNA polymerase I and that such enzymatic detection of the double-stranded DNA is a sensitive approach enabling in-situ detection of both the nuclear and mitochondrial genomes in formaldehyde-fixed human cells." . . "Strunin, Dmytro" . "The study describes the method of a sensitive detection of double-stranded DNA molecules in situ. It is based on the oxidative attack on the deoxyribose moiety by copper(I) in the presence of oxygen. We have shown previously that the oxidative attack leads to the formation of frequent gaps in DNA. Here we have demonstrated that the gaps can be utilized as the origins for an efficient synthesis of complementary labeled strands by DNA polymerase I and that such enzymatic detection of the double-stranded DNA is a sensitive approach enabling in-situ detection of both the nuclear and mitochondrial genomes in formaldehyde-fixed human cells."@en . "Ligasov\u00E1, Anna" . "15110" . "Koberna, Karel" . . "RIV/61989592:15110/13:33143922" . "PLoS One" . "[2D524CFE2273]" . "58826" . . "A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA"@en . . . . . "8" . "1932-6203" . "6"^^ . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA" . "000320576400163" . .