"15110" . . "Zp\u016Fsob produkce synchronizovan\u00FDch adherentn\u011B rostouc\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED a p\u0159\u00EDstroj pro prov\u00E1d\u011Bn\u00ED tohoto zp\u016Fsobu"@cs . . "RIV/61989592:15110/11:10225365!RIV12-MSM-15110___" . . . "\u00DA\u0159ad pr\u016Fmyslov\u00E9ho vlastnictv\u00ED" . "P(ED0030/01/01), S" . "Zp\u016Fsob produkce synchronizovan\u00FDch adherentn\u011B rostouc\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED, jeho\u017E podstata spo\u010D\u00EDv\u00E1 v tom, \u017Ee kultiva\u010Dn\u00ED l\u00E1hev (4) napln\u011Bn\u00E1 kultiva\u010Dn\u00EDm m\u00E9diem (41) s vysazenou adherentn\u00ED bun\u011B\u010Dnou lini\u00ED se podrobuje v inkub\u00E1toru po dobu 6 a\u017E 30 hodin p\u0159i standardn\u00EDch kultiva\u010Dn\u00EDch podm\u00EDnk\u00E1ch st\u00E1l\u00FDm vibrac\u00EDm o velikosti 1 a\u017E 100 kmit\u016F/sec zp\u016Fsobuj\u00EDc\u00EDm vyplavov\u00E1n\u00ED slab\u011B adheruj\u00EDc\u00EDch mitotick\u00FDch bun\u011Bk do kultiva\u010Dn\u00EDho m\u00E9dia (41) a iniciaci inhibice proliferace u t\u011Bchto bun\u011Bk, a po stanoven\u00E9m \u010Dasov\u00E9m \u00FAseku se vyplaven\u00E1 suspenzn\u00ED frakce bun\u011Bk odebere k vysazen\u00ED do nov\u00E9 kultiva\u010Dn\u00ED l\u00E1hve nebo ke zpracov\u00E1n\u00ED pro anal\u00FDzu. P\u0159\u00EDstroj pro realizaci zp\u016Fsobu sest\u00E1v\u00E1 z pohybliv\u00E9 plo\u0161iny (1), kter\u00E1 je jednak voln\u011B zav\u011B\u0161ena na nepohybliv\u00E9m podstavci (3), jednak je vybavena \u00FAchyty (10) pro uchycen\u00ED kultiva\u010Dn\u00ED l\u00E1hve (4) a jednak je k n\u00ED p\u0159ipevn\u011Bn vibr\u00E1tor (5) propojen\u00FD kabelem (6) s regul\u00E1torem (7) vibrac\u00ED ulo\u017Een\u00E9m v boxu (8), kter\u00FD je sou\u010D\u00E1st\u00ED podstavce (3) a uvnit\u0159 kter\u00E9ho je ulo\u017Een bateriov\u00FD zdroj (9)."@cs . "Prague" . . "[AEDDF8239EFD]" . "2"^^ . . "B\u00E1rtek, Ji\u0159\u00ED" . "Univerzita Palack\u00E9ho, Olomouc, CZ" . . . . . "mammalian cells; adherent cells; cell cycle; cell growth synchronization"@en . "In the present invention, there is disclosed a process for preparing synchronized adherently growing cell lines wherein the preparation process of the invention is characterized in that a culturing bottle (4) filled with a culturing medium (41) with a planted adherent cell line is subjected within an incubator and for a period of 6 to 30 hours at standard culturing conditions to permanent vibrations with the magnitude of 1 to 100 oscillations/sec causing elutriation of weakly adhering mitotic cells into the culture medium (41) and initiation of inhibition of proliferation of these cells. After lapse of the determined time interval, the elutriated suspension mass of cell fraction is taken for planting in a new culturing bottle or for analysis processing.; An apparatus for making the above-described process consists of a movable platform (1), which is both freely suspended on a stationary stand (3) and provided with mountings (10) for attaching the a culturing bottle (4). A vibrator (5) connected through the mediation of a cable (6) with a vibration controller (7) mounted within a box (8) forming a part of the stand (3) is attached to said movable platform (1) wherein a battery source is placed within said box (8), too."@en . "243650" . . . "2"^^ . . "Mistr\u00EDk, Martin" . . . . "Zp\u016Fsob produkce synchronizovan\u00FDch adherentn\u011B rostouc\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED, jeho\u017E podstata spo\u010D\u00EDv\u00E1 v tom, \u017Ee kultiva\u010Dn\u00ED l\u00E1hev (4) napln\u011Bn\u00E1 kultiva\u010Dn\u00EDm m\u00E9diem (41) s vysazenou adherentn\u00ED bun\u011B\u010Dnou lini\u00ED se podrobuje v inkub\u00E1toru po dobu 6 a\u017E 30 hodin p\u0159i standardn\u00EDch kultiva\u010Dn\u00EDch podm\u00EDnk\u00E1ch st\u00E1l\u00FDm vibrac\u00EDm o velikosti 1 a\u017E 100 kmit\u016F/sec zp\u016Fsobuj\u00EDc\u00EDm vyplavov\u00E1n\u00ED slab\u011B adheruj\u00EDc\u00EDch mitotick\u00FDch bun\u011Bk do kultiva\u010Dn\u00EDho m\u00E9dia (41) a iniciaci inhibice proliferace u t\u011Bchto bun\u011Bk, a po stanoven\u00E9m \u010Dasov\u00E9m \u00FAseku se vyplaven\u00E1 suspenzn\u00ED frakce bun\u011Bk odebere k vysazen\u00ED do nov\u00E9 kultiva\u010Dn\u00ED l\u00E1hve nebo ke zpracov\u00E1n\u00ED pro anal\u00FDzu. P\u0159\u00EDstroj pro realizaci zp\u016Fsobu sest\u00E1v\u00E1 z pohybliv\u00E9 plo\u0161iny (1), kter\u00E1 je jednak voln\u011B zav\u011B\u0161ena na nepohybliv\u00E9m podstavci (3), jednak je vybavena \u00FAchyty (10) pro uchycen\u00ED kultiva\u010Dn\u00ED l\u00E1hve (4) a jednak je k n\u00ED p\u0159ipevn\u011Bn vibr\u00E1tor (5) propojen\u00FD kabelem (6) s regul\u00E1torem (7) vibrac\u00ED ulo\u017Een\u00E9m v boxu (8), kter\u00FD je sou\u010D\u00E1st\u00ED podstavce (3) a uvnit\u0159 kter\u00E9ho je ulo\u017Een bateriov\u00FD zdroj (9)." . "Zp\u016Fsob produkce synchronizovan\u00FDch adherentn\u011B rostouc\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED a p\u0159\u00EDstroj pro prov\u00E1d\u011Bn\u00ED tohoto zp\u016Fsobu" . "Production process of synchronized adherently growing cell lines and apparatus for making the same"@en . "RIV/61989592:15110/11:10225365" . . . . . "Production process of synchronized adherently growing cell lines and apparatus for making the same"@en . . "2011-07-20+02:00"^^ . . "Zp\u016Fsob produkce synchronizovan\u00FDch adherentn\u011B rostouc\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED a p\u0159\u00EDstroj pro prov\u00E1d\u011Bn\u00ED tohoto zp\u016Fsobu"@cs . "302682" . . . "Zp\u016Fsob produkce synchronizovan\u00FDch adherentn\u011B rostouc\u00EDch bun\u011B\u010Dn\u00FDch lini\u00ED a p\u0159\u00EDstroj pro prov\u00E1d\u011Bn\u00ED tohoto zp\u016Fsobu" . .