"I, Z(MSM6198959216)" . . "ELISA; Stroke; Visinin like protein 1; VILIP-1"@en . . . . "RIV/61989592:15110/11:10223863!RIV12-MSM-15110___" . . "3"^^ . "5"^^ . . . "Visinin like protein 1 (VILIP-1) is a newly discovered CNS-abundant protein which has shown promise in experimental studies, for early stroke diagnosis. However, to date there is no clinical study that has measured VILIP-1 in sera as a marker of stroke. Aim. To develop an assay for the determination of VILIP-1 in human serum, and to investigate its clinical relevance as a marker of ischemic stroke. A new sandwich ELISA was developed, introduced and clinically tested. Mean spiking recovery was 98%. The mean recovery for dilution linearity was 93%. The limit of detection of the assay was 0.01 mcg/l; the intraassay and interassay coefficient of variation (CV) were always less than 10%. The study was approved by the Ethics Commission of the Hospital \u0160ternberk, Czech Republic. A total of 17 healthy individuals (9 men and 8 women, age 64.0 +- 13.0) and 16 individuals with ischemic stroke (10 men and 6 women, age 63.0+-11.5) were recruited for our study. The criteria of stroke were proposed by the National Czech Standard. All individuals had blood samples drawn, and VILIP-1 analysis and CT and/or MRI were performed. VILIP-1 serum level significantly differentiated healthy subjects from patients with stroke (P{0.01). All individuals with stroke had VILIP-1 serum values higher than } 0.05 mcg/l, healthy had values below this value. The diagnostic efficacy of serum VILIP-1 was very significant (sensitivity 100%, specificity 100% at 0.093 mcg/l VILIP-1 serum values, AUC 1.0 (CI 0.93-1.0, P{0.01), Chi-squared in the frequency table was 33 (P{0.01). We have introduced a new analytical tool for the study of VILIP-1. Our results support the hypothesis that serum VILIP-1 may be associated with ischemic stroke. The ELISA VILIP-1 assay offers a new research tool for the diagnosis and pathophysiology of stroke and other CNS diseases."@en . "RIV/61989592:15110/11:10223863" . "DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY"@en . "Karp\u00ED\u0161ek, Michal" . "1213-8118" . "155" . "DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY"@en . "4"^^ . . "DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY" . "193885" . "Stejskal, David" . "Biomedical Papers-Olomouc" . "\u0160vest\u00E1k, Marek" . "CZ - \u010Cesk\u00E1 republika" . "15110" . . . "Visinin like protein 1 (VILIP-1) is a newly discovered CNS-abundant protein which has shown promise in experimental studies, for early stroke diagnosis. However, to date there is no clinical study that has measured VILIP-1 in sera as a marker of stroke. Aim. To develop an assay for the determination of VILIP-1 in human serum, and to investigate its clinical relevance as a marker of ischemic stroke. A new sandwich ELISA was developed, introduced and clinically tested. Mean spiking recovery was 98%. The mean recovery for dilution linearity was 93%. The limit of detection of the assay was 0.01 mcg/l; the intraassay and interassay coefficient of variation (CV) were always less than 10%. The study was approved by the Ethics Commission of the Hospital \u0160ternberk, Czech Republic. A total of 17 healthy individuals (9 men and 8 women, age 64.0 +- 13.0) and 16 individuals with ischemic stroke (10 men and 6 women, age 63.0+-11.5) were recruited for our study. The criteria of stroke were proposed by the National Czech Standard. All individuals had blood samples drawn, and VILIP-1 analysis and CT and/or MRI were performed. VILIP-1 serum level significantly differentiated healthy subjects from patients with stroke (P{0.01). All individuals with stroke had VILIP-1 serum values higher than } 0.05 mcg/l, healthy had values below this value. The diagnostic efficacy of serum VILIP-1 was very significant (sensitivity 100%, specificity 100% at 0.093 mcg/l VILIP-1 serum values, AUC 1.0 (CI 0.93-1.0, P{0.01), Chi-squared in the frequency table was 33 (P{0.01). We have introduced a new analytical tool for the study of VILIP-1. Our results support the hypothesis that serum VILIP-1 may be associated with ischemic stroke. The ELISA VILIP-1 assay offers a new research tool for the diagnosis and pathophysiology of stroke and other CNS diseases." . . . "[E00C877CE815]" . "Sporov\u00E1, Lenka" . "DETERMINATION OF SERUM VISININ LIKE PROTEIN-1 AND ITS POTENTIAL FOR THE DIAGNOSIS OF BRAIN INJURY DUE TO THE STROKE - A PILOT STUDY" . . . . . "3" . . . . .