"Je\u017Eek, Jan" . "253157" . . "3"^^ . "P(GA303/08/0658), Z(AV0Z50110509), Z(AV0Z50200510), Z(MSM6198959216)" . "Dehydrosilybin; Respiration uncoupling; Protonophore; Mitochondria; Cardiomyocytes; Reactive oxygen species"@en . . . "Ga\u017E\u00E1k, Radek" . . "10"^^ . . . . "15110" . . "Modriansk\u00FD, Martin" . "Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism" . "Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism"@en . . "Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism"@en . . "Jab\u016Frek, Martin" . . . . . "0145-479X" . "6" . . "GABRIELOV\u00C1, Eva" . . . "RIV/61989592:15110/10:10212701" . . "Vost\u00E1lov\u00E1, Jitka" . "RIV/61989592:15110/10:10212701!RIV11-GA0-15110___" . "K\u0159en, Vladim\u00EDr" . . . "Journal of Bioenergetics and Biomembranes" . "[72348C66B072]" . . . . "Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism" . "Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging. Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. When investigating the cytoprotective effects of these two compounds in the primary cell cultures of neonatal rat cardiomyocytes, we noted the ability of dehydrosilybin to de-energize the cells by monitoring JC-1 fluorescence. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols." . "7"^^ . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "42" . . "Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging. Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. When investigating the cytoprotective effects of these two compounds in the primary cell cultures of neonatal rat cardiomyocytes, we noted the ability of dehydrosilybin to de-energize the cells by monitoring JC-1 fluorescence. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. We infer that the apparent uncoupler-like activity of dehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols."@en .