. "3"^^ . "1"^^ . . "Kr\u00E1lov\u00E1, D." . . "Finding of clonal chromosomal aberrations in plasma cells is considered as one of the most important and independent prognostic factor in patients with multiple myeloma (MM). The aim of the study was to assess the frequency and prognostic value of the most common chromosomal aberrations in a homogenous series of patients with newly diagnosed multiple myeloma enrolled to the phase III trial CMG 2002. In this study 208 patients enrolled until December 31, 2005 were included. All patients underwent single autologous bone marrow transplantation according to protocol of the trial after 4xVAD induction chemotherapy and were randomized after transplantation to two maintanance/consolidation treatment arms. All patients gave written informed consent to the proposed therapeutic procedure and to the provision of samples for research purposes. Cytoplasmic Ig-FISH (cIg-FISH) analyses were done on plasma cells labeled by the Amca Anti-Human kappa-chain, Amca Anti-Human lambda-chain and Amca Anti-goat IgG monoclonal"@en . . "IT - Italsk\u00E1 republika" . "Molecular cytogenetic study of immunofluorescently labeled plasma cells and prognostic significance of clonal chromosomal aberrations in 208 patients with multiple myeloma"@en . "Tajtlov\u00E1, J." . "Z(MSM6198959205)" . . "N\u011Bmec, P." . "Michalov\u00E1, K." . "Molekul\u00E1rn\u011B cytogenetick\u00E1 anal\u00FDza imunofluorescen\u010Dn\u011B zna\u010Den\u00FDch plazmatick\u00FDch bun\u011Bk u pacient\u016F s mnoho\u010Detn\u00FDm myelomem ve studii CMG 2002"@cs . "Oltov\u00E1, A." . "Pavli\u0161tov\u00E1, L." . "RIV/61989592:15110/08:00007542!RIV09-MSM-15110___" . "Za v\u00FDznamn\u00FD prognostick\u00FD faktor je u nemocn\u00FDch s mnoho\u010Detn\u00FDm myelomem (MM) pova\u017Eov\u00E1n n\u00E1lez klon\u00E1ln\u00EDch chromozomov\u00FDch aberac\u00ED v plazmatick\u00FDch bu\u0148k\u00E1ch n\u00E1lez klon\u00E1ln\u00EDch chromozomov\u00FDch aberac\u00ED v plazmatick\u00FDch bu\u0148k\u00E1ch v dob\u011B stanoven\u00ED diagn\u00F3zy. K nej\u010Dast\u011Bj\u0161\u00EDm n\u00E1lez\u016Fm apt\u0159\u00ED delece RB1 genu v oblasti 13q14 a/nebo ztr\u00E1ta cel\u00E9ho chromozomu 13 (st\u0159edn\u00ED a\u017E \u0161patn\u00E1 progn\u00F3za) a translogace IgH genu v oblasti 14q32 (v\u011Bt\u0161inou \u0161patn\u00E1 progn\u00F3za). K nep\u0159\u00EDzniv\u00FDm prognsotick\u00FDm faktor\u016Fm pat\u0159\u00ED i amplifikace oblasti 1q21 a delece tumor supresorov\u00E9ho genu p53. c\u00EDlem proejktu bylo zji\u0161t\u011Bn\u00ED frekvence a prognostick\u00E9ho v\u00FDznamu specifick\u00FDch chromozomov\u00FDch zm\u011Bn u nemocn\u00FDch s MM za\u0159azen\u00FDch v letech 2003-2006 do klinick\u00E9 studie CMG 2002. Konven\u010Dn\u00ED cytogenetickou anal\u00FDzou a anal\u00FDzou imunnofluorescen\u010Dn\u011B znam\u010Den\u00FDch plazmocyt\u016F metodou cIg-FISH jsme vy\u0161et\u0159ili 208 nov\u011B diagnostikovan\u00FDch pacient\u016F s MM. U 52,9 % nemocn\u00FDch jsme detekovali aberace chromozomu 13, p\u0159estavby IgH genu jsme prok\u00E1zali u 58,6 % pacient\u016F. Deleci gen\u016F p53 a zisk chromoz"@cs . "Molekul\u00E1rn\u011B cytogenetick\u00E1 anal\u00FDza imunofluorescen\u010Dn\u011B zna\u010Den\u00FDch plazmatick\u00FDch bun\u011Bk u pacient\u016F s mnoho\u010Detn\u00FDm myelomem ve studii CMG 2002"@cs . . "Jaro\u0161ov\u00E1, Marie" . "RIV/61989592:15110/08:00007542" . "Haematologica: the hematology journal" . . "Filkov\u00E1, H." . "Zemanov\u00E1, Zuzana" . "93" . . "Holzerov\u00E1, Milena" . "12"^^ . . "multiple myeloma (MM); cytoplasmic Ig-FISH (cIg-FISH) analyse"@en . . "Kugl\u00EDk, P." . "Molecular cytogenetic study of immunofluorescently labeled plasma cells and prognostic significance of clonal chromosomal aberrations in 208 patients with multiple myeloma" . . "Molecular cytogenetic study of immunofluorescently labeled plasma cells and prognostic significance of clonal chromosomal aberrations in 208 patients with multiple myeloma"@en . . "380327" . "Molecular cytogenetic study of immunofluorescently labeled plasma cells and prognostic significance of clonal chromosomal aberrations in 208 patients with multiple myeloma" . "0390-6078" . "1" . . "[47D0035BAB08]" . "15110" . . . . "Finding of clonal chromosomal aberrations in plasma cells is considered as one of the most important and independent prognostic factor in patients with multiple myeloma (MM). The aim of the study was to assess the frequency and prognostic value of the most common chromosomal aberrations in a homogenous series of patients with newly diagnosed multiple myeloma enrolled to the phase III trial CMG 2002. In this study 208 patients enrolled until December 31, 2005 were included. All patients underwent single autologous bone marrow transplantation according to protocol of the trial after 4xVAD induction chemotherapy and were randomized after transplantation to two maintanance/consolidation treatment arms. All patients gave written informed consent to the proposed therapeutic procedure and to the provision of samples for research purposes. Cytoplasmic Ig-FISH (cIg-FISH) analyses were done on plasma cells labeled by the Amca Anti-Human kappa-chain, Amca Anti-Human lambda-chain and Amca Anti-goat IgG monoclonal" . "Balc\u00E1rkov\u00E1, Jana" .