. . . . "Inhibitory serinov\u00FDch prote\u00E1z N-\u03B1-Tosyl-L-Lysinyl-Chloromethylketone (TLCK) a N-Tosyl-L-Phenylalaninyl-Chloromethylketone (TPCK) neinhibuj\u00ED aktivaci kasp\u00E1zy-3 a kasp\u00E1zy-7 u bun\u011Bk vystaven\u00FDch p\u016Fsoben\u00ED pro-apoptotick\u00FDch podn\u011Bt\u016F"@cs . . "Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) do not inhibit caspase-3 and caspase-7 processing in cells exposed to pro-apoptotic inducing stimuli" . "We recently demonstrated that TLCK and TPCK could act as potent but nonspecific inhibitors of mature caspases [Frydrych and Mlejnek [2008] J Cell Biochem 103:1646-1656]. The question whether TLCK and TPCK inhibit simultaneously caspase activation and/or processing remained, however, open. In this article, we demonstrated that TPCK even enhanced caspase-3 and caspase-7 processing although it substantially inhibited caspase-3 and caspase-7 enzymatic (DEVDase) activity in HL-60 cells exposed to various cell death inducing stimuli. Under the same conditions, TLCK had no effect or affected caspase-3 and caspase-7 processing marginally depending on cell treatment used. Importantly, TLCK substantially inhibited caspase-3 and caspase-7 enzymatic (DEVDase) activity irrespectively to the treatment used. Interestingly, treatment of cells with toxic concentrations of TPCK alone was accompanied by full caspase-3 and -7 processing even if it induced necrosis. In contrast, treatment of cells with concentrations of T"@en . . "Z(MSM6198959216)" . "Mlejnek, Petr" . "6"^^ . "RIV/61989592:15110/08:00007391!RIV09-MSM-15110___" . . "SERINE PROTEASE INHIBITORS; MATURE CASPASES; CASPASE-3 PROCESSING; CASPASE-7 PROCESSING; APOPTOSIS; NECROSIS"@en . "6" . "105" . "V ned\u00E1vn\u00E9 dob\u011B jsme uk\u00E1zali, \u017Ee TLCK a TPCK p\u016Fsob\u00ED jako \u00FA\u010Dinn\u00E9, ale nespecifick\u00E9 inhibitory aktivovan\u00FDch kasp\u00E1z [Frydrych and Mlejnek [2008] J Cell Biochem 103:1646-1656]. Nicm\u00E9n\u011B ot\u00E1zka zda-li TLCK a TPCK sou\u010Dasn\u011B inhibuj\u00ED aktivaci kasp\u00E1z z\u016Fstala otev\u0159en\u00E1."@cs . "15110" . . "[65746941525D]" . "RIV/61989592:15110/08:00007391" . . "Journal of Cellular Biochemistry" . . "We recently demonstrated that TLCK and TPCK could act as potent but nonspecific inhibitors of mature caspases [Frydrych and Mlejnek [2008] J Cell Biochem 103:1646-1656]. The question whether TLCK and TPCK inhibit simultaneously caspase activation and/or processing remained, however, open. In this article, we demonstrated that TPCK even enhanced caspase-3 and caspase-7 processing although it substantially inhibited caspase-3 and caspase-7 enzymatic (DEVDase) activity in HL-60 cells exposed to various cell death inducing stimuli. Under the same conditions, TLCK had no effect or affected caspase-3 and caspase-7 processing marginally depending on cell treatment used. Importantly, TLCK substantially inhibited caspase-3 and caspase-7 enzymatic (DEVDase) activity irrespectively to the treatment used. Interestingly, treatment of cells with toxic concentrations of TPCK alone was accompanied by full caspase-3 and -7 processing even if it induced necrosis. In contrast, treatment of cells with concentrations of T" . . . "2"^^ . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . . "394474" . . . . "Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) do not inhibit caspase-3 and caspase-7 processing in cells exposed to pro-apoptotic inducing stimuli"@en . "2"^^ . . "Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) do not inhibit caspase-3 and caspase-7 processing in cells exposed to pro-apoptotic inducing stimuli" . "Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) do not inhibit caspase-3 and caspase-7 processing in cells exposed to pro-apoptotic inducing stimuli"@en . "0730-2312" . "Inhibitory serinov\u00FDch prote\u00E1z N-\u03B1-Tosyl-L-Lysinyl-Chloromethylketone (TLCK) a N-Tosyl-L-Phenylalaninyl-Chloromethylketone (TPCK) neinhibuj\u00ED aktivaci kasp\u00E1zy-3 a kasp\u00E1zy-7 u bun\u011Bk vystaven\u00FDch p\u016Fsoben\u00ED pro-apoptotick\u00FDch podn\u011Bt\u016F"@cs . "Frydrych, Ivo" .