"Chemical Modification of Trypsin"@en . "RIV/61989592:15110/08:00007302" . "15110" . . . . . . . . "1"^^ . "Chemical Modification of Trypsin"@en . . "RIV/61989592:15110/08:00007302!RIV09-MSM-15110___" . "\u0160tosov\u00E1, Ta\u0165\u00E1na" . . . "P(2E06029)" . "1"^^ . . "359733" . . . "[DBDE2ABE6722]" . . "P\u0159edlo\u017Een\u00E1 diserta\u010Dn\u00ED pr\u00E1ce je zam\u011B\u0159en\u00E1 na p\u0159\u00EDpravu konjug\u00E1t\u016F a deriv\u00E1t\u016F hov\u011Bz\u00EDho trypsinu (BT) odoln\u00FDch v\u016F\u010Di autol\u00FDze a vykazuj\u00EDc\u00EDch vysokou katalitickou aktivitu p\u0159i vy\u0161\u0161\u00EDch teplot\u00E1ch. Prim\u00E1rn\u00ED aminoskupiny BT Pzbytky lysin\u016F) byly modifikov\u00E1ny AMP \u010Di ATP, formaldehydem a molekulami sacharid\u016F. pro modifikaci argininov\u00FDch zbytk\u016F byl zvolen phenylglyoxalem a formaldehydem (FF-BT), lactosoumelibiosou-modifikovan\u00FD BT (LAC-BT), maltosou-modifikovan\u00FD BT (MAL-BT), melibiosou-modifikovan\u00FD-BT (MEL-BT), maltotriosou-modifikovan\u00FD BT (MAT-BT), rafinosou-modifikovan\u00FD BT (RAF-BT), rafinosou-modifikovan\u00FD BT nav\u00EDc s modifikovan\u00FDmi zbytky arginin\u016F reakc\u00ED s 2,3-butanedionem (RAFR-BT), stachyosou-modifikovan\u00FD BT (STA-BT), \u03B1-cyclodextrinem-modifikovan\u00FD BT (ACD-BT) a \u03B2-cyclodextrinem-modifikovan\u00FD BT (BCD-BT)."@cs . "P\u0159edlo\u017Een\u00E1 diserta\u010Dn\u00ED pr\u00E1ce je zam\u011B\u0159en\u00E1 na p\u0159\u00EDpravu konjug\u00E1t\u016F a deriv\u00E1t\u016F hov\u011Bz\u00EDho trypsinu (BT) odoln\u00FDch v\u016F\u010Di autol\u00FDze a vykazuj\u00EDc\u00EDch vysokou katalitickou aktivitu p\u0159i vy\u0161\u0161\u00EDch teplot\u00E1ch. Prim\u00E1rn\u00ED aminoskupiny BT Pzbytky lysin\u016F) byly modifikov\u00E1ny AMP \u010Di ATP, formaldehydem a molekulami sacharid\u016F. pro modifikaci argininov\u00FDch zbytk\u016F byl zvolen phenylglyoxalem a formaldehydem (FF-BT), lactosoumelibiosou-modifikovan\u00FD BT (LAC-BT), maltosou-modifikovan\u00FD BT (MAL-BT), melibiosou-modifikovan\u00FD-BT (MEL-BT), maltotriosou-modifikovan\u00FD BT (MAT-BT), rafinosou-modifikovan\u00FD BT (RAF-BT), rafinosou-modifikovan\u00FD BT nav\u00EDc s modifikovan\u00FDmi zbytky arginin\u016F reakc\u00ED s 2,3-butanedionem (RAFR-BT), stachyosou-modifikovan\u00FD BT (STA-BT), \u03B1-cyclodextrinem-modifikovan\u00FD BT (ACD-BT) a \u03B2-cyclodextrinem-modifikovan\u00FD BT (BCD-BT)." . . "The presented Ph.D. thesis is focused on development of autolysis-resistant bovine trypsin (BT) conjugates and derivates showing high catalytic activity at elevated temperatures. BT was modified by coupling of AMP or ATP, formaldehyde, sugars to primary amino groups (lysine residues) in the enzyme molecule and phenylglyoxal to modify arginine residues. The following BT conjugates were prepared: BT conjugated with AMP (AMP-BT) and ATP (ATP-BT), BT modify by phenylglyoxal and formaldehyde (FF-BT), lactose-modified BT (LAC-BT), maltose-modified-BT (MAL-BT), mellibiose-modified-BT (MEL-BT), maltotriose-modified BT (MAT-BT), raffinose-modified BT (STA-BT), \u03B1-cyclodextrin-modified BT (ACD-BT) and \u03B2-cyclodextrin-modified BT (BCD-BT)."@en . . "trypsin; modification; formaldehyde; phenylglyoxal"@en . "Chemick\u00E1 modifikace trypsinu"@cs . "Chemick\u00E1 modifikace trypsinu"@cs . "Chemick\u00E1 modifikace trypsinu" . "Chemick\u00E1 modifikace trypsinu" .