"Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells"@en . "Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells"@en . "Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells" . . . . . "RIV/61989592:15110/08:00006999!RIV09-MSM-15110___" . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "[32D1930B1FC6]" . . . . "Ulrichov\u00E1, Jitka" . . "Chelerythrin a dihydrochelerythrin zp\u016Fsobuj\u00ED v bu\u0148k\u00E1ch HL-60 zastaven\u00ED bun\u011B\u010Dn\u00E9ho cyklu v G1 f\u00E1zi a bimod\u00E1ln\u00ED bun\u011B\u010Dnou smrt"@cs . . . "Dole\u017Eel, Petr" . "22" . . . "5"^^ . . . . "Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells" . . "10"^^ . . "Toxicology in Vitro" . "3"^^ . "Modriansk\u00FD, Martin" . . "P(GP303/06/P193), Z(MSM6198959216)" . . . "Kvartern\u00ED benzo[c]fenanthridinov\u00FD alkaloid chelerythrin vykazuje \u0159adu biologick\u00FDch aktivit v\u010Detn\u011B cytotoxicity v\u016F\u010Di norm\u00E1ln\u00EDm a n\u00E1dorov\u00FDm bu\u0148k\u00E1m. M\u00E9n\u011B je v\u0161ak zn\u00E1mo o biologick\u00E9 aktivit\u011B dihydrochelerythrinu, produktu redukce chelerythrinu. My jsme se zab\u00FDvali srovn\u00E1n\u00EDm cytotoxicity chelerythrinu a dihydrochelerythrinu v leukemick\u00FDch bu\u0148k\u00E1ch HL-60. P\u0159i inkubaci po dobu 4 h chelerythrin sni\u017Eoval \u017Eivotnost bun\u011Bk, p\u0159i\u010Dem\u017E hodnota IC50 z\u00EDskan\u00E1 MTT metodou byla 2,6 ?mol/l. Dihydrochelerythrin byl m\u00E9n\u011B cytotoxick\u00FD, kdy\u017E p\u0159i koncentraci 20 ?mol/l sn\u00ED\u017Eil po 24 h \u017Eivotnost pouze na 53%. Pokles \u017Eivotnosti vlivem obou alkaloid\u016F byl doprov\u00E1zen apoptotick\u00FDmi zm\u011Bnami zahrnuj\u00EDc\u00EDmi ztr\u00E1tu mitochondri\u00E1ln\u00EDho membr\u00E1nov\u00E9ho poteci\u00E1lu, aktivaci kaspasy-9 a -3 a degradaci DNA. Pouze chelerythrin nav\u00EDc zv\u00FD\u0161il tak\u00E9 aktivitu kaspasy-8. Indukce apoptosy a nekrosy vlivem chelerythrinu a dihydrochelerythrinu byla potvrzena pr\u016Ftokovou cytometri\u00ED vyu\u017E\u00EDvaj\u00EDc\u00ED zna\u010Den\u00ED bun\u011Bk annexinem V a propidium jodidem. Oba alkaloidy tak\u00E9 zp\u016Fsobov"@cs . "359698" . . "A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of dihydrochelerythrine, a product of chelerythrine reduction. We examined the cytotoxicity of chelerythrine and dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not dihydrochelerythrine, elevated the activity of caspase-8. A d" . "0887-2333" . "RIV/61989592:15110/08:00006999" . "Chelerythrin a dihydrochelerythrin zp\u016Fsobuj\u00ED v bu\u0148k\u00E1ch HL-60 zastaven\u00ED bun\u011B\u010Dn\u00E9ho cyklu v G1 f\u00E1zi a bimod\u00E1ln\u00ED bun\u011B\u010Dnou smrt"@cs . "4" . "Vrba, Ji\u0159\u00ED" . "Apoptosis; Cell cycle; Chelerythrine; Cytotoxicity; Dihydrochelerythrine; HL-60 cells; Mitochondrial membrane potential"@en . "Vi\u010Dar, Jaroslav" . "15110" . "A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of dihydrochelerythrine, a product of chelerythrine reduction. We examined the cytotoxicity of chelerythrine and dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not dihydrochelerythrine, elevated the activity of caspase-8. A d"@en .