. "1213-8118" . . "Very modest changes in mRNA stability can aff ect critical points in cellular energy pathways. The aim of this study was to investigate the impact of energy abundant substrates on peroxisome proliferator-activated receptors (PPARs) and PPAR-. coactivators (PGCs) mRNA?s steady-state levels. Quantitative RT-PCR study was performed to assess the effect of zero or normal (5 mmol/l) glucose and/or oleic acid (0.3 mmol/l) on mRNA levels of (PPARs) (PGCs) in HepG2 cells. PGC-1alfa mRNA was significantly upregulated in glucose deprived cells (123 % of the control level; p < 0.05), while PGC-1\u00DF mRNA was significantly enhanced in oleate-fed cells (134 % and 160 % of control levels for zero glucose plus oleate and normal glucose plus oleate, respectively; p < 0.05) during the 0.5 h incubation. Upon the 4 h incubation, PPAR-gamma1 and PGC-1alfa mRNAs were significantly elevated in cells lacking glucose (142 % and 163 % of control levels, respectively; p < 0.05). Oleate significantly suppressed PPAR-al" . . . "9"^^ . "RIV/61989592:15110/07:00004428" . . "mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells"@en . "Bogdanov\u00E1, Kate\u0159ina" . . . "RIV/61989592:15110/07:00004428!RIV08-MSM-15110___" . . "Velmi mal\u00E9 zm\u011Bny mRNA stability mohou ovlivnit rozhoduj\u00EDc\u00ED body bun\u011B\u010Dn\u00FDch energetick\u00FDch drah. C\u00EDlem t\u00E9to studie bylo prozkoumat vliv energeticky bohat\u00FDch substr\u00E1t\u016F na st\u00E1l\u00E9 hladiny mRNA receptor\u016F aktivovan\u00FDch prolifer\u00E1tory peroxisom\u016F (PPARs) a PPAR-gamma koktiv\u00E1tor\u016F (PGCs). Byla provedena kvantitativn\u00ED RT-PCR studie pro vyhodnocen\u00ED vlivu nulov\u00E9 nebo norm\u00E1ln\u00ED (5 mmol/l) gluk\u00F3zy a kyseliny olejov\u00E9 (0,3 mmol/l) na hladiny mRNA (PPARs) (PGCs) v hepatomov\u00FDch HepG2 bu\u0148k\u00E1ch. Hladiny mRNA. PGC-1alfa byly signifikantn\u011B zv\u00FD\u0161en\u00E9 v bu\u0148k\u00E1ch ovlivn\u011Bn\u00FDch nedostatkem gluk\u00F3zy (123% kontroln\u00ED hodnoty; p < 0,05), zat\u00EDmco hladiny mRNA PGC 1beta byly signifikantn\u011B zv\u00FD\u0161en\u00E9 v bu\u0148k\u00E1ch ovlivn\u011Bn\u00FDch kyselinou olejovou (134% a 160% kontroln\u00ED hodnoty v bu\u0148k\u00E1ch ovlivn\u011Bn\u00FDch nulovou gluk\u00F3zou s kyselinou olejovou a ovlivn\u011Bn\u00FDch norm\u00E1ln\u00ED gluk\u00F3zou s kyselinou olejovou; p < 0,05) b\u011Bhem 0,5 h inkubace. Po 4 h inkubaci byly signifikantn\u011B zv\u00FD\u0161en\u00E9 hladiny mRNA PPAR-gamma a PGC-1alfa v bu\u0148k\u00E1ch s nulovou gluk\u00F3zou (142% a 163% kontroln\u00ED"@cs . "Biomedical Papers" . "Very modest changes in mRNA stability can aff ect critical points in cellular energy pathways. The aim of this study was to investigate the impact of energy abundant substrates on peroxisome proliferator-activated receptors (PPARs) and PPAR-. coactivators (PGCs) mRNA?s steady-state levels. Quantitative RT-PCR study was performed to assess the effect of zero or normal (5 mmol/l) glucose and/or oleic acid (0.3 mmol/l) on mRNA levels of (PPARs) (PGCs) in HepG2 cells. PGC-1alfa mRNA was significantly upregulated in glucose deprived cells (123 % of the control level; p < 0.05), while PGC-1\u00DF mRNA was significantly enhanced in oleate-fed cells (134 % and 160 % of control levels for zero glucose plus oleate and normal glucose plus oleate, respectively; p < 0.05) during the 0.5 h incubation. Upon the 4 h incubation, PPAR-gamma1 and PGC-1alfa mRNAs were significantly elevated in cells lacking glucose (142 % and 163 % of control levels, respectively; p < 0.05). Oleate significantly suppressed PPAR-al"@en . "Hladiny mRNA receptor\u016F aktivovan\u00FDch prolifer\u00E1tory peroxisom\u016F a jejich koaktiv\u00E1tor\u016F jsou ovlivn\u011Bny nedostatkem gluk\u00F3zy a kyselinou olejovou v lidsk\u00FDch hepatomov\u00FDch HepG2 bu\u0148k\u00E1ch"@cs . . . . "5"^^ . "mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells" . . . "5"^^ . . "Poczatkov\u00E1, Hana" . . "Vesel\u00FD, Jaroslav" . . "435525" . . "CZ - \u010Cesk\u00E1 republika" . "Uherkov\u00E1, Lenka" . "237-245" . . "mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells"@en . "[5BE7B3107823]" . . "Starvation; Glucose; Oleic acid; Peroxisome proliferator-activated receptor (PPAR); PPAR-gama coactivator (PGC); mRNA stability; Superinduction; HepG2 cells"@en . "Hladiny mRNA receptor\u016F aktivovan\u00FDch prolifer\u00E1tory peroxisom\u016F a jejich koaktiv\u00E1tor\u016F jsou ovlivn\u011Bny nedostatkem gluk\u00F3zy a kyselinou olejovou v lidsk\u00FDch hepatomov\u00FDch HepG2 bu\u0148k\u00E1ch"@cs . "mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells" . . "P(OC 153), Z(MSM6198959205)" . "151" . . . . . . . "2" . "Rypka, Miroslav" . "15110" .