. . "748" . "JAK2 genotyping (Real-time allelic discrimination) reveals rare JAK2 V617F-positive erythroid colonies in pediatric patients with essential thrombocythemia"@en . . . "Divok\u00FD, Vladim\u00EDr" . "Genotypov\u00E1n\u00ED JAK2 odhalilo u pediatrick\u00FDch pacient\u016F s trombocytemi\u00ED vz\u00E1cn\u011B se vyskytuj\u00EDc\u00ED erythroidn\u00ED kolonie pozitivn\u00ED na JAK2 V617F"@cs . "JAK2 genotyping (Real-time allelic discrimination) reveals rare JAK2 V617F-positive erythroid colonies in pediatric patients with essential thrombocythemia"@en . "Horv\u00E1thov\u00E1, Monika" . . "Genotypov\u00E1n\u00ED JAK2 odhalilo u pediatrick\u00FDch pacient\u016F s trombocytemi\u00ED vz\u00E1cn\u011B se vyskytuj\u00EDc\u00ED erythroidn\u00ED kolonie pozitivn\u00ED na JAK2 V617F"@cs . "\u010Cmejlov\u00E1, Jana" . . "Childhood essential thrombocythemia (ET) is a very rare disease belonging to a group of Bcr-Abl negative myeloproliferative disorders. Our study involved 15 pediatric ET patients diagnosed at the age of 6-17 years. Monoclonal hematopoiesis was detected only in one patient, who is recently an adult (diagnosed at the age of 9). JAK2 V617F was detected in whole blood DNA by allele-specific (AS)-PCR and BsaXI restriction analysis. In our hands AS-PCR sensitivity reached the detection limit of one homozygous cell in 2000 normal cells. In addition, more sensitive AS-RT-PCR was performed on RNA isolated from granulocytes and platelets from peripheral blood. Only one patient was JAK2 V617F-positive as detected by these established protocols. In the clonal assay of hematopoietic progenitors, most patients showed hypersensitivity to erythropoietin (Epo) in vitro with the growth of Epo-independent erythroid colonies (EECs) in some of them. Over 300 myeloid colonies were picked and analyzed by real-time allelic d"@en . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . . . "RIV/61989592:15110/07:00004017" . . . "748"^^ . . . . "Childhood thrombocythemia; EPO; mutation"@en . . "B\u011Bli\u010Dkov\u00E1, M." . . . "Soln\u00E1, Ren\u00E1ta" . "Veselovsk\u00E1, Jitka" . "RIV/61989592:15110/07:00004017!RIV08-MZ0-15110___" . "Posp\u00ED\u0161ilov\u00E1, Dagmar" . . . "P(NR9471), Z(MSM6198959205), Z(MSM6198959216)" . "427917" . "[1A1AFFFF711F]" . "JAK2 genotyping (Real-time allelic discrimination) reveals rare JAK2 V617F-positive erythroid colonies in pediatric patients with essential thrombocythemia" . . "JAK2 genotyping (Real-time allelic discrimination) reveals rare JAK2 V617F-positive erythroid colonies in pediatric patients with essential thrombocythemia" . "11" . "Pekov\u00E1, So\u0148a" . "Star\u00FD, J." . "8"^^ . "0006-4971" . . "15110" . "Blood" . . "D\u011Btsk\u00E1 esenci\u00E1ln\u00ED trombocyt\u00E9mie (ET) pat\u0159\u00ED mezi vz\u00E1cn\u00E9 onemocn\u011Bn\u00ED ze skupiny Bcr-Abl negativn\u00EDch myeloproliferativn\u00EDch onemocn\u011Bn\u00ED. Na\u0161e studie zahrnuje 15 d\u011Btsk\u00FDch pacient\u016F s ET, kte\u0159\u00ED byli diagnostikov\u00E1n\u00ED ve v\u011Bku 6-17 let ..."@cs . "10"^^ . "\u010Cmejla, Radek" . . . "110" . "Childhood essential thrombocythemia (ET) is a very rare disease belonging to a group of Bcr-Abl negative myeloproliferative disorders. Our study involved 15 pediatric ET patients diagnosed at the age of 6-17 years. Monoclonal hematopoiesis was detected only in one patient, who is recently an adult (diagnosed at the age of 9). JAK2 V617F was detected in whole blood DNA by allele-specific (AS)-PCR and BsaXI restriction analysis. In our hands AS-PCR sensitivity reached the detection limit of one homozygous cell in 2000 normal cells. In addition, more sensitive AS-RT-PCR was performed on RNA isolated from granulocytes and platelets from peripheral blood. Only one patient was JAK2 V617F-positive as detected by these established protocols. In the clonal assay of hematopoietic progenitors, most patients showed hypersensitivity to erythropoietin (Epo) in vitro with the growth of Epo-independent erythroid colonies (EECs) in some of them. Over 300 myeloid colonies were picked and analyzed by real-time allelic d" .