. . "Dvo\u0159\u00E1k, Zden\u011Bk" . . "Molecular and Cellular Endocrinology" . "Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes" . "RIV/61989592:15110/07:00003561" . "Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes" . . "Cellular signaling by glucocorticoid receptor and aryl hydrocarbon receptor is restricted by microtubules interfering agents (MIAs). This leads to down-regulation of drug metabolizing enzymes and drug interactions. Here we investigated the effects of all-trans-retinoic acid (ATRA) and MIAs, i.e. colchicine, nocodazole and taxol on the regulation of retinoic acid receptor (RAR) genes in primary cultures of rat hepatocytes. ATRA (1 \u03BCM) down-regulated RAR\u03B1 and RAR\u03B3 mRNAs (decrease 23% and 41%, respectively) whereas it up-regulated RAR\u03B2 mRNA (4.3-fold induction). All MIAs diminished the expression of RARs in dose-dependent manner; the potency of MIAs increased in order NOC < COL < TAX and the extent of inhibition increased in order RAR\u03B1 < RAR\u03B3 < RAR\u03B2. The levels of RAR\u03B1 protein were decreased by both MIAs and ATRA. The effects of ATRA were reversed by proteasome inhibitor MG-132, implying ligand-dependent RAR\u03B1 degradation. In contrast, the effects o"@en . . "15110" . . "Vrzal, Radim" . . "0303-7207" . . . "8"^^ . "267" . . "6"^^ . "Ondkov\u00E1, Slavom\u00EDra" . "Exprese, stabilita proteinu a transkrip\u010Dn\u00ED aktivity receptor\u016F pro kyselinu retinovou v prim\u00E1rn\u00EDch potkan\u00EDch hepatocytech jsou ovlivn\u011Bny l\u00E1tkami naru\u0161uj\u00EDc\u00EDmi mikrotubuly a all-trans retinovou kyselinou"@cs . "Bun\u011B\u010Dn\u00E1 signalizace glukokortikoidn\u00EDm receptorem a aryl uhlovod\u00EDkov\u00FDm receptorem je naru\u0161ena l\u00E1tkami interferuj\u00EDc\u00EDmi s mikrotubuly (MIA). N\u00E1sledkem je down-regulace enzym\u016F metabolizuj\u00EDc\u00EDch l\u00E9\u010Diva a l\u00E9kov\u00E9 interakce. Zde jsme zkoumali \u00FA\u010Dinky kyseliny all-trans retinov\u00E9 (ATRA) a MIA (tj. kolchicinu, nokodazolu, taxolu) na regulaci gen\u016F receptor\u016F pro kyselinu retinovou (RAR) v prim\u00E1rn\u00EDch kultur\u00E1ch potkan\u00EDch hepatocyt\u016F. ATRA zp\u016Fsobila pokles RARa a RARg mRNA (pokles 23% a 41%), zat\u00EDmco obsah RARb mRNA byl zv\u00FD\u0161en (4,3 kr\u00E1t). V\u0161echny MIA zp\u016Fsobily koncentra\u010Dn\u011B z\u00E1visl\u00FD pokles exprese RAR receptor\u016F; \u00FA\u010Dinnost MIA rostla v po\u0159ad\u00ED NOC "421422" . "Ulrichov\u00E1, Jitka" . "Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes"@en . . "Retinoic acid receptors; Cellular signaling; Microtubules; Rat hepatocytes"@en . . . "3"^^ . "RIV/61989592:15110/07:00003561!RIV08-MSM-15110___" . "[2B489E1011C9]" . "Exprese, stabilita proteinu a transkrip\u010Dn\u00ED aktivity receptor\u016F pro kyselinu retinovou v prim\u00E1rn\u00EDch potkan\u00EDch hepatocytech jsou ovlivn\u011Bny l\u00E1tkami naru\u0161uj\u00EDc\u00EDmi mikrotubuly a all-trans retinovou kyselinou"@cs . "89-96" . "Expression, protein stability and transcriptional activity of retinoic acid receptors are affected by microtubules interfering agents and all-trans-retinoic acid in primary rat hepatocytes"@en . . . "IE - Irsko" . . . "1-2" . "Macejov\u00E1, Dana" . . "P(GP303/04/P074), Z(MSM6198959216)" . . . "Cellular signaling by glucocorticoid receptor and aryl hydrocarbon receptor is restricted by microtubules interfering agents (MIAs). This leads to down-regulation of drug metabolizing enzymes and drug interactions. Here we investigated the effects of all-trans-retinoic acid (ATRA) and MIAs, i.e. colchicine, nocodazole and taxol on the regulation of retinoic acid receptor (RAR) genes in primary cultures of rat hepatocytes. ATRA (1 \u03BCM) down-regulated RAR\u03B1 and RAR\u03B3 mRNAs (decrease 23% and 41%, respectively) whereas it up-regulated RAR\u03B2 mRNA (4.3-fold induction). All MIAs diminished the expression of RARs in dose-dependent manner; the potency of MIAs increased in order NOC < COL < TAX and the extent of inhibition increased in order RAR\u03B1 < RAR\u03B3 < RAR\u03B2. The levels of RAR\u03B1 protein were decreased by both MIAs and ATRA. The effects of ATRA were reversed by proteasome inhibitor MG-132, implying ligand-dependent RAR\u03B1 degradation. In contrast, the effects o" . "Brtko, J\u00FAlius" .